Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1865)
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Table of contents (21 protocols)
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Front Matter
Keywords
About this book
This volume explores techniques used to study and experiment with Xenopus in order to model or understand human disease. The chapters in this book cover topics such as implementation of CRISPR/Cas9 and TALEN in Xenopus from the design stage up to the genotyping stage; methods to explore generation of knock-in animals; cancer modelling and in vivo screening of congenital heart disease; electroporation; and phenotyping at the organismal level, cellular level, and proteome level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Xenopus: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this wide and developing field. This book will aid in keeping Xenopus at the forefront for biochemical, cell biological, and developmental studies and forward it as a preclinical disease model.
Editors and Affiliations
Bibliographic Information
Book Title: Xenopus
Book Subtitle: Methods and Protocols
Editors: Kris Vleminckx
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8784-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018
Hardcover ISBN: 978-1-4939-8783-2Published: 27 August 2018
Softcover ISBN: 978-1-4939-9391-8Published: 10 December 2019
eBook ISBN: 978-1-4939-8784-9Published: 27 August 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 302
Number of Illustrations: 11 b/w illustrations, 57 illustrations in colour
Topics: Human Genetics