Skip to main content
SpringerLink
Log in
Book cover

Morphogen Gradients

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Julien Dubrulle

    1. Integrated Microscopy Core, Baylor College of Medicine, Houston, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols for the study of morphogen gradients
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1863)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Visualization of Morphogen Gradients

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Analysis of Expression Gradients of Developmental Regulators in Arabidopsis thaliana Roots

      • María Florencia Ercoli, Rodrigo Vena, Camila Goldy, Javier F. Palatnik, Ramiro E. Rodríguez
      Pages 3-17

    3. Detection and Quantification of the Bicoid Concentration Gradient in Drosophila Embryos

      • Feng He, Honggang Wu, David Cheung, Jun Ma
      Pages 19-27

    4. Imaging Cytonemes in Drosophila Embryos

      • Lijuan Du, Sougata Roy
      Pages 29-45

    5. Measuring Dorsoventral Pattern and Morphogen Signaling Profiles in the Growing Neural Tube

      • Marcin Zagorski, Anna Kicheva
      Pages 47-63

  3. Analysis of Biophysical Properties and Functions of Morphogens

    1. Front Matter

      Pages 65-65
      Download chapter PDF

    2. Fluorescence Correlation and Cross-Correlation Spectroscopy in Zebrafish

      • Xue Wen Ng, Karuna Sampath, Thorsten Wohland
      Pages 67-105

    3. FRAP Analysis of Extracellular Diffusion in Zebrafish Embryos

      • Gary H. Soh, Patrick Müller
      Pages 107-124

    4. Generation of Ectopic Morphogen Gradients in the Zebrafish Blastula

      • Maraysa de Olivera-Melo, Peng-Fei Xu, Nathalie Houssin, Bernard Thisse, Christine Thisse
      Pages 125-141

    5. Polarizing Region Tissue Grafting in the Chick Embryo Limb Bud

      • Holly Stainton, Matthew Towers
      Pages 143-153

    6. Analysis of PLETHORA Gradient Formation by Secreted Peptides During Root Development

      • Hidefumi Shinohara, Yoshikatsu Matsubayashi
      Pages 155-164

    7. Live Imaging of mRNA Transcription in Drosophila Embryos

      • Carmina Angelica Perez-Romero, Huy Tran, Mathieu Coppey, Aleksandra M. Walczak, Cécile Fradin, Nathalie Dostatni
      Pages 165-182

    8. LiveFly: A Toolbox for the Analysis of Transcription Dynamics in Live Drosophila Embryos

      • Huy Tran, Carmina Angelica Perez-Romero, Teresa Ferraro, Cécile Fradin, Nathalie Dostatni, Mathieu Coppey et al.
      Pages 183-195

  4. Modeling of Morphogen Behaviors and Activities

    1. Front Matter

      Pages 197-197
      Download chapter PDF

    2. Discrete-State Stochastic Modeling of Morphogen Gradient Formation

      • Hamid Teimouri, Anatoly B. Kolomeisky
      Pages 199-221

    3. Simulation of Morphogen and Tissue Dynamics

      • Michael D. Multerer, Lucas D. Wittwer, Anna Stopka, Diana Barac, Christine Lang, Dagmar Iber
      Pages 223-250

    4. Mechanisms and Measurements of Scale Invariance of Morphogen Gradients

      • Yan Huang, David Umulis
      Pages 251-262

    5. Scaling of Morphogenetic Patterns

      • Manan’Iarivo Rasolonjanahary, Bakhtier Vasiev
      Pages 263-280

    6. Modelling Time-Dependent Acquisition of Positional Information

      • Laurent Jutras-Dubé, Adrien Henry, Paul François
      Pages 281-301
Back to top

Keywords

About this book

This detailed book presents methods focusing on the visualization of morphogen gradients, the analysis of their biophysical and biological properties, and the theoretical aspects underlying their functions. The study of morphogen gradients combines biophysics, cell and developmental biology, and applied mathematics approaches to understand their formation, their biological functions, and the prediction of their behavior in space and time, and this volume serves as a practical, hands-on guide to the field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and helpful, Morphogen Gradients: Methods and Protocols is an ideal collection for researchers in this vital area of study.

Editors and Affiliations

  • Integrated Microscopy Core, Baylor College of Medicine, Houston, USA

    Julien Dubrulle

Bibliographic Information

  • Book Title: Morphogen Gradients

  • Book Subtitle: Methods and Protocols

  • Editors: Julien Dubrulle

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8772-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8771-9Published: 16 October 2018

  • Softcover ISBN: 978-1-4939-9388-8Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8772-6Published: 15 October 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 306

  • Number of Illustrations: 7 b/w illustrations, 136 illustrations in colour

  • Topics: Cell Biology

Publish with us

Policies and ethics

Back to top

Search

Navigation