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X-Chromosome Inactivation

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1861)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Screening for Factors Involved in X Chromosome Inactivation Using Haploid ESCs

    • Asun Monfort, Giulio Di Minin, Anton Wutz
    Pages 1-18
  3. ChIRP-MS: RNA-Directed Proteomic Discovery

    • Ci Chu, Howard Y. Chang
    Pages 37-45
  4. Manipulation of Xist Imprinting in Mouse Preimplantation Embryos

    • Atsushi Fukuda, Akihiro Umezawa, Hidenori Akutsu
    Pages 47-53
  5. Live Imaging of Xist RNA

    • Osamu Masui, Edith Heard, Haruhiko Koseki
    Pages 67-72
  6. Visualization of Hidden Epitopes at the Inactive X Chromosome

    • Emma R. Stewart, Dawn Coverley
    Pages 103-112
  7. Chromosome Spread Analyses of Meiotic Sex Chromosome Inactivation

    • Kris G. Alavattam, Hironori Abe, Akihiko Sakashita, Satoshi H. Namekawa
    Pages 113-129
  8. Simultaneous RNA–DNA FISH in Mouse Preimplantation Embryos

    • Aristea Magaraki, Agnese Loda, Joost Gribnau, Willy M. Baarends
    Pages 131-147
  9. RNA-FISH and Immunofluorescence of Mouse Preimplantation and Postimplantation Embryos

    • Hirosuke Shiura, Yuka Sakata, Kuniya Abe, Takashi Sado
    Pages 161-176
  10. Experimental Analysis of Imprinted Mouse X-Chromosome Inactivation

    • Marissa Cloutier, Clair Harris, Srimonta Gayen, Emily Maclary, Sundeep Kalantry
    Pages 177-203
  11. X-Chromosome Inactivation and Escape from X Inactivation in Mouse

    • Wenxiu Ma, Giancarlo Bonora, Joel B. Berletch, Xinxian Deng, William S. Noble, Christine M. Disteche
    Pages 205-219
  12. Practical Analysis of Hi-C Data: Generating A/B Compartment Profiles

    • Hisashi Miura, Rawin Poonperm, Saori Takahashi, Ichiro Hiratani
    Pages 221-245
  13. Back Matter

    Pages 247-251

About this book

This book details recently developed technologies and conventionally employed cytological proceduresfor the study of X-Chromosome Inactivation. Chapters detail live imaging, bioinformatic methods, fluorescence in situ hybridization, and immunofluorescence, and procedures to optimize the study of molecular mechanism underlying X chromosome inactivation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, X-Chromosome Inactivation: Methods Protocols aims to be useful for researchers in the field of epigenetics, chromatin, noncoding RNA, and nuclear architecture.

Editors and Affiliations

  • Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara, Japan

    Takashi Sado

Bibliographic Information

  • Book Title: X-Chromosome Inactivation

  • Book Subtitle: Methods and Protocols

  • Editors: Takashi Sado

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8766-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8765-8Published: 15 September 2018

  • Softcover ISBN: 978-1-4939-9386-4Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8766-5Published: 14 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 251

  • Number of Illustrations: 10 b/w illustrations, 43 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access