Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1860)
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Table of contents (26 protocols)
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Front Matter
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Biophysics and Computational Analysis
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Front Matter
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Biochemistry
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Front Matter
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Functional Assays
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Front Matter
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Keywords
About this book
This book details multiple ways that soluble N-ethylmaleimide-sensitive factor attachment protein receptors( SNAREs) and their function are examined in the laboratory. The methods described in each chapter are described in detail so that novice as well as experienced researchers can explore the mechanisms of SNARE-mediated membrane fusion. Chapters guide readers through an overview of the basic properties of SNAREs, distribution and interaction with regulators of membrane fusion, activation of SNAREs in the priming stage by NSF/Sec18 and a-SNAP/Sec17, examining the structures and interactions of SNAREs, measuring the interactions of SNAREs, interactions of SNAREs, and post-translational modifications of SNAREs and how they affect function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, SNAREs: Methods and Protocols aims to be a valuable tool for all investigators interested in the field.Editors and Affiliations
Bibliographic Information
Book Title: SNAREs
Book Subtitle: Methods and Protocols
Editors: Rutilio Fratti
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8760-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019
Hardcover ISBN: 978-1-4939-8759-7Published: 14 October 2018
Softcover ISBN: 978-1-4939-9385-7Published: 10 December 2019
eBook ISBN: 978-1-4939-8760-3Published: 13 October 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 407
Number of Illustrations: 8 b/w illustrations, 66 illustrations in colour
Topics: Biochemistry, general