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Microbial Metabolomics

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Edward E.K. Baidoo

    1. Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, USA

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  • Includes cutting-edge methods and protocols for the study of microbial metabolomics
  • Provides step-by-step details essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1859)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Microbial Metabolomics: A General Overview

    • Edward E. K. Baidoo
    Pages 1-8

  3. Microbial Metabolomics, Metabolism, and Physiology

    1. Front Matter

      Pages 9-9
      Download chapter PDF

    2. Mass Spectrometry-Based Microbial Metabolomics: Techniques, Analysis, and Applications

      • Edward E. K. Baidoo, Veronica Teixeira Benites
      Pages 11-69

    3. Metabolomics: A Microbial Physiology and Metabolism Perspective

      • Chijioke J. Joshua
      Pages 71-94

  5. Current Analytical Techniques

    1. Front Matter

      Pages 153-153
      Download chapter PDF

    2. Targeted Metabolomics of Xylose-Fermenting Yeasts Based on Mass Spectrometry

      • Christiane Gonçalves Campos, José Antônio de Aquino Ribeiro, João Ricardo Moreira de Almeida, Betania Ferraz Quirino, Patrícia Verardi Abdelnur
      Pages 155-169

    3. Exploiting High-Resolution Mass Spectrometry for Targeted Metabolite Quantification and 13C-Labeling Metabolism Analysis

      • Zhucui Li, Yujing Li, Yinjie J. Tang, Wenqing Shui
      Pages 171-184

    4. Quantitative Profiling of Endogenous Metabolites Using Hydrophilic Interaction Liquid Chromatography–Tandem Mass Spectrometry (HILIC-MS/MS)

      • Attila Teleki, Ralf Takors
      Pages 185-207

    5. Liquid Chromatography and Mass Spectrometry Analysis of Isoprenoid Intermediates in Escherichia coli

      • Edward E. K. Baidoo, George Wang, Chijioke J. Joshua, Veronica Teixeira Benites, Jay D. Keasling
      Pages 209-224

    6. Determining the Mode of Action of Antimalarial Drugs Using Time-Resolved LC-MS-Based Metabolite Profiling

      • Simon A. Cobbold, Malcolm J. McConville
      Pages 225-239

    7. Use of Liquid Chromatography–Mass Spectrometry-Based Metabolomics to Identify Biomarkers of Tuberculosis

      • Juntuo Zhou, Yuxin Yin
      Pages 241-251

    8. Metabolomics Analysis of Leishmania by Capillary Electrophoresis and Mass Spectrometry

      • David Rojo, Coral Barbas, Ángeles López-Gonzálvez
      Pages 253-260
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Keywords

About this book

This detailed volume includes protocols that represent the breadth of microbial metabolomics approaches to both large-scale and small-scale experiments with intention of highlighting techniques that can be used for applications ranging from environmental microbiology to human disease. Utilizing mass spectrometry as their primary measurement tool, the chapters explore microbial metabolomics, metabolism and microbial physiology, metabolite sample preparation, current analytical techniques used to profile primary and secondary metabolites and lipids, as well as establishing data analysis workflows for targeted metabolomics, untargeted metabolomics, analysis of metabolic fluxes, and genome-scale models. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Microbial Metabolomics: Methods and Protocols serves as an ideal reference for both novice and advanced users and can be adapted to similar analytical platforms or customized to suit the needs of the researcher.

Editors and Affiliations

  • Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, Emeryville, USA

    Edward E.K. Baidoo

Bibliographic Information

  • Book Title: Microbial Metabolomics

  • Book Subtitle: Methods and Protocols

  • Editors: Edward E.K. Baidoo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8757-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-8756-6Published: 13 November 2018

  • eBook ISBN: 978-1-4939-8757-3Published: 12 November 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 351

  • Number of Illustrations: 33 b/w illustrations, 50 illustrations in colour

  • Topics: Microbiology

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