Editors:
- Includes cutting-edge methods and protocols for the study of recombinant protein expression
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1850)
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Table of contents (19 protocols)
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Front Matter
About this book
This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and convenient, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.
Editors and Affiliations
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Protein Production and Structure Core Facility (PPSCF), Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
David L. Hacker
Bibliographic Information
Book Title: Recombinant Protein Expression in Mammalian Cells
Book Subtitle: Methods and Protocols
Editors: David L. Hacker
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8730-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018
Hardcover ISBN: 978-1-4939-8729-0Published: 22 September 2018
Softcover ISBN: 978-1-4939-9377-2Published: 10 December 2019
eBook ISBN: 978-1-4939-8730-6Published: 21 September 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 311
Number of Illustrations: 8 b/w illustrations, 68 illustrations in colour
Topics: Protein Science