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  • © 2018

Plant Pathogenic Fungi and Oomycetes

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1848)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. Quantifying Re-association of a Facultative Endohyphal Bacterium with a Filamentous Fungus

    • David A. Baltrus, Joseph Spraker, A. Elizabeth Arnold
    Pages 1-11
  3. Characterizing Mycoviruses

    • Mahtab Peyambari, Marilyn J. Roossinck
    Pages 13-24
  4. Protocols for Investigating the Leaf Mycobiome Using High-Throughput DNA Sequencing

    • Shawn P. Brown, Devin R. Leopold, Posy E. Busby
    Pages 39-51
  5. Characterizing Small RNAs in Filamentous Fungi Using the Rice Blast Fungus, Magnaporthe oryzae, as an Example

    • Vidhyavathi Raman, Blake C. Meyers, Ralph A. Dean, Nicole M. Donofrio
    Pages 53-66
  6. Illuminating Phytophthora Biology with Fluorescent Protein Tags

    • Audrey M. V. Ah-Fong, Meenakshi Kagda, Howard S. Judelson
    Pages 119-129
  7. Methods for Transient Gene Expression in Wild Relatives of Potato

    • Sidrat Abdullah, Dennis Halterman
    Pages 131-138
  8. From Short Reads to Chromosome-Scale Genome Assemblies

    • Kyle Fletcher, Richard Michelmore
    Pages 151-197
  9. Application of the Cre/lox System to Construct Auxotrophic Markers for Quantitative Genetic Analyses in Fusarium graminearum

    • Lanelle R. Connolly, Allyson A. Erlendson, Corinne M. Fargo, Kendra K. Jackson, Morgan M. G. Pelker, Jacob W. Mazzola et al.
    Pages 235-263
  10. Protocol of Phytophthora capsici Transformation Using the CRISPR-Cas9 System

    • Zhiwen Wang, Brett M. Tyler, Xili Liu
    Pages 265-274
  11. Generating Gene Silenced Mutants in Phytophthora sojae

    • Yao Zhao, Yufei Li, Min Qiu, Wenbo Ma, Yuanchao Wang
    Pages 275-286
  12. Back Matter

    Pages 287-292

About this book

This book describes approaches for the analysis of plant pathogenic fungi that range from ecosystem composition to in vitro and wet-lab to computational analyses. Chapters detail bioinformatics protocols for genome assembly, transcriptome analysis and small RNA profiling, characterization of secondary metabolome, analysis of post-translational modification and localization of proteins, examination of virulence function and interaction with plants or other microorganisms, and targeted mutagenesis using various approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Plant Pathogenic Fungi and Oomycetes: Methods and Protocols aims to provide techniques developed through awide range of perspectives to facilitate research on a comprehensive understanding of these important filamentous pathogens.


Editors and Affiliations

  • Department of Microbiology and Plant Pathology, University of California Riverside, Riverside, USA

    Wenbo Ma

  • Oregon State University, Corvallis, USA

    Thomas Wolpert

Bibliographic Information

  • Book Title: Plant Pathogenic Fungi and Oomycetes

  • Book Subtitle: Methods and Protocols

  • Editors: Wenbo Ma, Thomas Wolpert

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8724-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8723-8Published: 04 September 2018

  • Softcover ISBN: 978-1-4939-9375-8Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8724-5Published: 04 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 292

  • Number of Illustrations: 14 b/w illustrations, 36 illustrations in colour

  • Topics: Plant Sciences

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access