Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1844)
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Table of contents (25 protocols)
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Front Matter
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Methods to Uncover the Mechanisms of Action for Enzymes that Assemble or Disassemble Poly-Ubiquitin Chains
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Front Matter
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Tools to Study Ubiquitylation
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Front Matter
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Structural Approaches as Applied to Enzymes that Participate in the Ubiquitin Proteasome System
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Front Matter
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Methods to Study 26S Proteasome Function
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Front Matter
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About this book
“This volume explores numerous techniques used to study the ubiquitin proteasome system. The chapters in this book are organized into five parts and cover topics such as determining the mechanisms of action for E2s, E3s, and DUB enzymes; the latest advances to study the formation of poly-ubiquitin chains as well as their linkage types; the binding partners of proteins in the UPS; methods for structure determination by x-ray crystallography, cryo electron microscopy and SAXS; screening assays to select for degrons or modulators of E3s and DUBs; proteomics approaches in the ubiquitin field and methods to study 26S proteasome function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and authoritative, The UbiquitinProteasome System: Methods and Protocols is a valuable resource for both experienced and novice scientists who are interested in expanding their knowledge in this field.
Editors and Affiliations
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Department of Biochemistry and Molecular Biology, Michael Smith Laboratories, University of British Columbia, Vancouver, Canada
Thibault Mayor
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Department of Chemistry and Biochemistry, University of Nevada Las Vegas, Las Vegas, USA
Gary Kleiger
Bibliographic Information
Book Title: The Ubiquitin Proteasome System
Book Subtitle: Methods and Protocols
Editors: Thibault Mayor, Gary Kleiger
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8706-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018
Hardcover ISBN: 978-1-4939-8705-4Published: 22 September 2018
Softcover ISBN: 978-1-4939-9371-0Published: 10 December 2019
eBook ISBN: 978-1-4939-8706-1Published: 21 September 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XV, 413
Number of Illustrations: 29 b/w illustrations, 39 illustrations in colour
Topics: Protein Science