Skip to main content
  • Book
  • © 2018

Vibrio Cholerae

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1839)

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
  2. Laboratory Culturing Techniques and Maintenance of Vibrio cholerae

    • Kyle D. Brumfield, Bailey M. Carignan, Mike S. Son
    Pages 1-9
  3. Genotypic and Phenotypic Assays to Distinguish Vibrio cholerae Biotype

    • Kyle D. Brumfield, Bailey M. Carignan, Mike S. Son
    Pages 11-28
  4. Metabolomics of Vibrio cholerae

    • Yusuke Minato, Jay S. Kirkwood, Claudia C. Häse
    Pages 45-51
  5. Chromatin Immunoprecipitation

    • Julio C. Ayala, Jorge A. Benitez, Anisia J. Silva
    Pages 65-75
  6. Isolation of Outer Membrane Vesicles Including Their Quantitative and Qualitative Analyses

    • Paul Kohl, Franz G. Zingl, Thomas O. Eichmann, Stefan Schild
    Pages 117-134
  7. Proteomics of Vibrio cholerae

    • Ryszard A. Zielke
    Pages 171-178
  8. Back Matter

    Pages 179-180

About this book

This volume looks at the importance of V. cholerae as an infection agent, and explores the latest techniques used to study it. The chapters in this book cover topics such as the biology of V. cholerae; laboratory maintenance and culturing; various animals models developed to explain pathogenesis and factors for environmental survival; and holistic approaches that include proteomics, metabolomics, and high-throughput screening. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting-edge and authoritative, Vibrio cholerae: Methods and Protocols is a valuable resource for both novice microbiologists who want to focus their studies on V. cholerae, and experienced researchers and teachers who want to improve their knowledge and skills in this growing field.


Editors and Affiliations

  • Department of Pharmaceutical Sciences, College of Pharmacy, Oregon State University, Corvallis, USA

    Aleksandra E. Sikora

Bibliographic Information

  • Book Title: Vibrio Cholerae

  • Book Subtitle: Methods and Protocols

  • Editors: Aleksandra E. Sikora

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8685-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8684-2Published: 26 July 2018

  • Softcover ISBN: 978-1-4939-9365-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8685-9Published: 25 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 180

  • Number of Illustrations: 8 b/w illustrations, 19 illustrations in colour

  • Topics: Microbiology

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access