Skip to main content
  • Book
  • © 2018

Targeted Drug Delivery

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1831)

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
  2. Drug Carrier Design

    1. Front Matter

      Pages 1-1
    2. Synthesis of Cationic Polymer Libraries for Gene Delivery Using Diglycidyl Ethers

      • Jacob Elmer, Thrimoorthy Potta, Kaushal Rege
      Pages 3-16
    3. Generation of Ultra-Small PLGA Nanoparticles by Sequential Centrifugation

      • Xingwang Wu, Jiangbing Zhou, Toral R. Patel
      Pages 17-24
    4. Construction of Bacteria-Based Cargo Carriers for Targeted Cancer Therapy

      • Mahama A. Traore, Ali Sahari, Bahareh Behkam
      Pages 25-35
    5. Production of Extracellular Vesicles Loaded with Therapeutic Cargo

      • Tek N. Lamichhane, Steven M. Jay
      Pages 37-47
  3. Passive and Active Targeting Methods

    1. Front Matter

      Pages 69-69
    2. Exploiting Phage Display for Development of Novel Cellular Targeting Strategies

      • William Marsh, Amanda Witten, Sarah E. Stabenfeldt
      Pages 71-94
    3. CD44 Targeted Lipid Nanoparticles for MicroRNA Therapy

      • Stephen L. Hayward, Srivatsan Kidambi
      Pages 95-109
    4. In Vitro Validation of Targeting and Comparison to Mathematical Modeling

      • Jill M. Steinbach-Rankins, Michael R. Caplan
      Pages 121-141
  4. Measuring Delivery and Efficacy

    1. Front Matter

      Pages 143-143
    2. Enzyme-Linked Immunosorbent Assay to Quantify Targeting Molecules on Nanoparticles

      • Rachel S. Riley, Jilian R. Melamed, Emily S. Day
      Pages 145-157
    3. Utilizing the Lung as a Model to Study Nanoparticle-Based Drug Delivery Systems

      • Dylan K. McDaniel, Veronica M. Ringel-Scaia, Sheryl L. Coutermarsh-Ott, Irving C. Allen
      Pages 179-190
    4. Non-Enzymatic Tissue Homogenization for Biodistribution Analysis

      • Danielle M. DiPerna, Alesia V. Prakapenka, Eugene P. Chung, Rachael W. Sirianni
      Pages 191-199
    5. Methods and Study Designs for Characterizing the Pharmacokinetics and Pharmacodynamics of Carrier-Mediated Agents

      • Allison N. Schorzman, Andrew T. Lucas, John R. Kagel, William C. Zamboni
      Pages 201-228
  5. Back Matter

    Pages 229-230

About this book

This volume looks at the latest techniques used to enable therapeutic molecules to be targeted for site-specific delivery. The chapters in this book discuss topics such as approaches to express biologically derived and synthetic nanocarriers; strategies to assist in nanocarrier targeting to specific cells and tissues; and methods for evaluating delivery and efficiency for new classes of agents. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and comprehensive, Targeted Drug Delivery: Methods and Protocols is a valuable resource for anyone interested in understanding the diversity of methods used in this evolving field.


Editors and Affiliations

  • Vivian L. Smith Department of Neurosurgery, University of Texas Health Science, Center at Houston, Houston, USA

    Rachael W. Sirianni

  • Department of Mechanical Engineering, Virginia Tech, Blacksburg, USA

    Bahareh Behkam

Bibliographic Information

  • Book Title: Targeted Drug Delivery

  • Book Subtitle: Methods and Protocols

  • Editors: Rachael W. Sirianni, Bahareh Behkam

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8661-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8659-0Published: 27 July 2018

  • Softcover ISBN: 978-1-4939-9357-4Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8661-3Published: 26 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 230

  • Number of Illustrations: 13 b/w illustrations, 39 illustrations in colour

  • Topics: Pharmacology/Toxicology

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access