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Serpins

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Alexandra Lucas

    1. Biodesign Center for Personalized Diagnostics and Center for Immunotherapy Vaccines and Virotherapy, Arizona State University, Tempe, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1826)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Overview of Serpins and Their Roles in Biological Systems

    • Alexandra Lucas, Jordan R. Yaron, Liqiang Zhang, Sriram Ambadapadi
    Pages 1-7

  3. Methods for Determining and Understanding Serpin Structure and Function: X-Ray Crystallography

    • Brian P. Mahon, Robert McKenna
    Pages 9-39

  4. Serpin Phage Display: The Use of a T7 System to Probe Reactive Center Loop Libraries with Different Serine Proteinases

    • Lucas R. de Souza, Benjamin M. Scott, Varsha Bhakta, David A. Donkor, Darian L. Perruzza, William P. Sheffield
    Pages 41-64

  5. Kinetic Measurement of Serpin Inhibitory Activity by Real-Time Fluorogenic Biochemical Assay

    • Jordan R. Yaron, Sriram Ambadapadi, Liqiang Zhang, Alexandra Lucas
    Pages 65-71

  6. Methods for Identifying Virus-Derived Serpins

    • Masmudur M. Rahman
    Pages 73-86

  7. In Vitro Approaches for the Assessment of Serpin Polymerization

    • Emma L. K. Elliston, David A. Lomas, James A. Irving
    Pages 87-107

  8. Cellular Models for the Serpinopathies

    • Annamaria Fra, Emanuela D’Acunto, Mattia Laffranchi, Elena Miranda
    Pages 109-121

  9. Binding of Serpins to Immobilized Phospholipids and Phospholipids in Suspension

    • Felix C. Wahlmüller, Judit Mihaly-Bison, Margarethe Geiger
    Pages 123-132

  10. Viral Serpin Reactive Center Loop (RCL) Peptides: Design and Testing

    • Liqiang Zhang, Jordan R. Yaron, Sriram Ambadapadi, Alexandra Lucas
    Pages 133-142

  11. In Vivo Analysis of Alpha-1-Antitrypsin Functions in Autoimmune Disease Models

    • Sihong Song, Yuanqing Lu, Ahmed S. Elshikha
    Pages 143-155

  12. Analysis of In Vivo Serpin Functions in Models of Inflammatory Vascular Disease

    • Hao Chen, Sriram Ambadapadi, Erbin Dai, Liying Liu, Jordan R. Yaron, Liqiang Zhang et al.
    Pages 157-182

  13. Gene Delivery of Alpha-1-Antitrypsin Using Recombinant Adeno-Associated Virus (rAAV)

    • Sihong Song, Yuanqing Lu
    Pages 183-196

  14. Serpins in Venous Thrombosis and Venous Thrombus Resolution

    • Subhradip Mukhopadhyay, Tierra A. Johnson, Rajabrata Sarkar, Toni M. Antalis
    Pages 197-211

  15. Next-Generation Sequencing Library Preparation for 16S rRNA Microbiome Analysis After Serpin Treatment

    • Juan Maldonado, Jordan R. Yaron, Liqiang Zhang, Alexandra Lucas
    Pages 213-221

  16. Methods for Assessing Serpins as Neuroprotective Therapeutics

    • Jacek M. Kwiecien
    Pages 223-235

  17. Adeno-Associated Virus Delivery of Viral Serpins for Ocular Diseases: Design and Validation

    • Cristhian J. Ildefonso, Alfred S. Lewin
    Pages 237-254

  18. Serpins: Development for Therapeutic Applications

    • Alexandra Lucas, Jordan R. Yaron, Liqiang Zhang, Colin Macaulay, Grant McFadden
    Pages 255-265

  19. Back Matter

    Pages 267-276
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Keywords

About this book

This volume explores the latest methods used to study and define serpin molecular structure, basic protease inhibition, serpin targets, and the roles of serpin in biology and disease using animal models. The chapters in this book cover topics such as crystallography and phage display, peptide design, phospholipid binding, and thrombus formation to microbiome analysis and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Cutting edge and authoritative, Serpins: Methods and Protocols is a valuable resource for researchers and scientists interested in learning more about this evolving field.


Editors and Affiliations

  • Biodesign Center for Personalized Diagnostics and Center for Immunotherapy Vaccines and Virotherapy, Arizona State University, Tempe, USA

    Alexandra Lucas

Bibliographic Information

  • Book Title: Serpins

  • Book Subtitle: Methods and Protocols

  • Editors: Alexandra Lucas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8645-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8644-6Published: 08 September 2018

  • Softcover ISBN: 978-1-4939-9352-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8645-3Published: 07 September 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 276

  • Number of Illustrations: 12 b/w illustrations, 33 illustrations in colour

  • Topics: Protein Science

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