Skip to main content
  • Book
  • © 2018

miRNA Biogenesis

Methods and Protocols

  • Includes cutting-edge methods and protocols for the study of miRNA biogenesis
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1823)

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
  2. High-Throughput Characterization of Primary microRNA Transcripts

    • Tsung-Cheng Chang, Joshua T. Mendell
    Pages 1-9
  3. Identifying Pri-miRNA Transcription Start Sites

    • Georgios Georgakilas, Nikos Perdikopanis, Artemis G. Hatzigeorgiou
    Pages 11-31
  4. Metabolic Pulse-Chase RNA Labeling for pri-miRNA Processing Dynamics

    • Annita Louloupi, Ulf Andersson Vang Ørom
    Pages 33-41
  5. Purification of Microprocessor-Associated Factors

    • Ross A. Cordiner, Sara Macias
    Pages 51-62
  6. Inhibiting Pri-miRNA Processing with Target Site Blockers

    • Annita Louloupi, Ulf Andersson Vang Ørom
    Pages 63-68
  7. MicroRNA Analysis Using the Quantitative Real-Time PCR Reaction

    • Marta Kotlarek, Anna Kubiak, Krystian Jażdżewski, Anna Wójcicka
    Pages 69-85
  8. MicroRNA Analysis Using Next-Generation Sequencing

    • Monika Kolanowska, Anna Kubiak, Krystian Jażdżewski, Anna Wójcicka
    Pages 87-101
  9. Identification of microRNA Precursor-Associated Proteins

    • Thomas Treiber, Nora Treiber, Gunter Meister
    Pages 103-114
  10. Analysis of 3′ End Modifications in microRNAs by High-Throughput Sequencing

    • Madalena M. Reimão-Pinto, Angela M. Rodrigues-Viana, Stefan L. Ameres
    Pages 115-139
  11. Uncovering the Stability of Mature miRNAs by 4-Thio-Uridine Metabolic Labeling

    • Matteo J. Marzi, Francesco Nicassio
    Pages 141-152
  12. Detection of microRNA-Target Interactions by Chimera PCR (ChimP)

    • James P. Broughton, Amy E. Pasquinelli
    Pages 153-165
  13. High-Quality Overlapping Paired-End Reads for the Detection of A-to-I Editing on Small RNA

    • Josephine Galipon, Rintaro Ishii, Soh Ishiguro, Yutaka Suzuki, Shinji Kondo, Mariko Okada-Hatakeyama et al.
    Pages 167-183
  14. Method for Detection of miRNAs in Non-Model Organisms with Unreported Database

    • Parveen Bansal, Ashish Kumar, Sudhir Chandna, Malika Arora, Renu Bansal
    Pages 197-208
  15. Detection and Verification of Mammalian Mirtrons by Northern Blotting

    • Mohammad Farid Zia, Alex S. Flynt
    Pages 209-219
  16. Detecting Agotrons in Ago CLIPseq Data

    • Thomas B. Hansen
    Pages 221-232
  17. Back Matter

    Pages 233-238

About this book

This detailed volume collects chapters that seek to expand our knowledge of molecular events and extended molecular networks in gene regulation. The contents explore numerous aspects of miRNA biogenesis, a sophisticated series of events that assure a finely tuned regulation of miRNA expression and activity. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, miRNA Biogenesis: Methods and Protocols aims to contribute to the further development of knowledge and application of miRNA biogenesis, both in the basic research lab and in the clinic. ​


Editors and Affiliations

  • Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark

    Ulf Andersson Vang Ørom

Bibliographic Information

  • Book Title: miRNA Biogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Ulf Andersson Vang Ørom

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8624-8

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8623-1Published: 30 June 2018

  • Softcover ISBN: 978-1-4939-9345-1Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8624-8Published: 29 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 238

  • Number of Illustrations: 13 b/w illustrations, 45 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 119.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 159.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access