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Mouse Oocyte Development

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Marie-Hélène Verlhac

    1. CIRB, Collège de France, UMR7241, INSERM-U1050, Paris, France

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  2. Marie-Emilie Terret

    1. CIRB, Collège de France, UMR7241, INSERM-U1050, Paris, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1818)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
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  2. Growth In Vitro of Granulosa Cell–Oocyte Complexes of the Mouse

    • Stephany El-Hayek, Qin Yang, Hugh J. Clarke
    Pages 1-11

  3. In Vitro Culture of Mouse Oocytes for Meiotic Maturation

    • Ewa Borsuk, Jacek Z. Kubiak
    Pages 13-21

  4. In Vitro Maturation and Culture of Human Oocytes

    • Jennifer R. Gruhn, Stine Gry Kristensen, Claus Yding Andersen, Eva R. Hoffmann
    Pages 23-30

  5. Pronuclear Transfer in Human Oocytes

    • Louise Hyslop
    Pages 31-36

  6. In Vitro Fertilization of Mouse Oocyte

    • Agathe Chaigne
    Pages 37-42

  7. Profiling Maternal mRNA Translation During Oocyte Development

    • Joao P. Sousa Martins, Marco Conti
    Pages 43-50

  8. Transcriptome Profiling of Single Mouse Oocytes

    • Maud Borensztein, Laurène Syx, Nicolas Servant, Edith Heard
    Pages 51-65

  9. Immunofluorescence Technique to Detect Subcellular Structures Critical to Oocyte Maturation

    • Cecilia S. Blengini, Karen Schindler
    Pages 67-76

  10. Immunofluorescence Staining of K-Fibers in Mouse Oocytes Using Cold Fixation

    • Jessica Greaney, Zhe Wei, Hayden Homer
    Pages 77-87

  11. Triple-Color Live Imaging of Mouse Oocytes

    • Aurélien Courtois, Petr Solc, Tomoya S. Kitajima
    Pages 89-97

  12. Detection of Separase Activity Using a Cleavage Sensor in Live Mouse Oocytes

    • Elvira Nikalayevich, Nora Bouftas, Katja Wassmann
    Pages 99-112

  13. Manipulating Cohesin Levels in Live Mouse Oocytes

    • Anna Szydłowska, Sabrina Ladstätter, Kikuë Tachibana
    Pages 113-128

  14. Optogenetic Manipulation of Mouse Oocytes

    • Takashi Akera, David M. Chenoweth, Michael A. Lampson
    Pages 129-135

  15. Monitoring Microtubule Dynamics in the Mouse Egg Using Photoactivatable-GFP-Tubulin

    • Greg FitzHarris
    Pages 137-144

  16. Photoactivation of Actin in Mouse Oocyte

    • Maria Almonacid
    Pages 145-151

  17. Laser Ablation of Microtubule–Chromosome Attachment in Mouse Oocytes

    • Isma Bennabi, Marion Manil-Ségalen
    Pages 153-161

  18. Micropipette Aspiration of Oocytes to Assess Cortical Tension

    • Janice P. Evans, Douglas N. Robinson
    Pages 163-171

  19. Back Matter

    Pages 173-174
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Keywords

About this book

This book details methods on various aspects of the very final stages of mouse oocyte development. Chapters guide the reader through in vitro growth of follicles, production of a fully-grown competent mouse oocyte, meiosis resumption, analysis of spindle assembly and chromosome segregation, analysis of the oocyte and early embryo transcriptome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Mouse Oocyte Development: Methods and Protocols aims to help scientists with the set up both live and fix experiments required to study the process of oocyte meiotic maturation.

Reviews

“Each chapter presents not only detailed and reproducible methods (troubleshooting included) but also nice images and instructive figures providing non-verbal information necessary to better understand how to deal with this ‘fantastic little laboratory of molecular biology’ that is the oocyte.” (Manuela Monti, European Journal of Histochemistry, Vol. 62 (04), October, 2018)

Editors and Affiliations

  • CIRB, Collège de France, UMR7241, INSERM-U1050, Paris, France

    Marie-Hélène Verlhac, Marie-Emilie Terret

Bibliographic Information

  • Book Title: Mouse Oocyte Development

  • Book Subtitle: Methods and Protocols

  • Editors: Marie-Hélène Verlhac, Marie-Emilie Terret

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8603-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8602-6Published: 01 July 2018

  • Softcover ISBN: 978-1-4939-9340-6Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8603-3Published: 30 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 174

  • Number of Illustrations: 13 b/w illustrations, 30 illustrations in colour

  • Topics: Cell Biology

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