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Plant Cell Culture Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Víctor M. Loyola-Vargas

    1. Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Mexico

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  2. Neftalí Ochoa-Alejo

    1. Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Mexico

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  • Includes cutting-edge methods protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1815)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xv
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  2. Introduction

    1. Front Matter

      Pages 1-1
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    2. An Introduction to Plant Tissue Culture: Advances and Perspectives

      • Victor M. Loyola-Vargas, Neftalí Ochoa-Alejo
      Pages 3-13

  3. Cell Culture the Fundaments

    1. Front Matter

      Pages 15-15
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    2. Micropropagation in the Twenty-First Century

      • Jean Carlos Cardoso, Lee Tseng Sheng Gerald, Jaime A. Teixeira da Silva
      Pages 17-46

    3. Cellular and Morpho-histological Foundations of In Vitro Plant Regeneration

      • Diego Ismael Rocha, Lorena Melo Vieira, Andréa Dias Koehler, Wagner Campos Otoni
      Pages 47-68

    4. Bacterial Endophytes in Plant Tissue Culture: Mode of Action, Detection, and Control

      • Mona Quambusch, Traud Winkelmann
      Pages 69-88

    5. Digital Photography as a Tool of Research and Documentation in Plant Tissue Culture

      • Victor Gaba, Yehudit Tam, Danny Shavit, Benjamin Steinitz
      Pages 89-101

    6. Selection of Molecular Markers for the Estimation of Somaclonal Variation

      • Octavio Martínez
      Pages 103-129

    7. Plant Tissue Culture: A Battle Horse in the Genome Editing Using CRISPR/Cas9

      • Víctor M. Loyola-Vargas, Randy N. Avilez-Montalvo
      Pages 131-148

  4. Protocols

    1. Front Matter

      Pages 149-149
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    2. Micropropagation of Agave Species

      • Benjamín Rodríguez-Garay, José Manuel Rodríguez-Domínguez
      Pages 151-159

    3. Protocol for the Micropropagation of Coconut from Plumule Explants

      • Luis Sáenz, José Luis Chan, María Narvaez, Carlos Oropeza
      Pages 161-170

    4. Micropropagation of Yucca Species

      • Yessica López-Ramírez, Alejandra Palomeque-Carlín, Lucía Isabel Chávez Ortiz, Ma. de Lourdes de la Rosa-Carrillo, Eugenio Pérez-Molphe-Balch
      Pages 171-177

    5. Auxin Immunolocalization in Coffea canephora Tissues

      • Ruth E. Márquez-López, Ángela Ku-González, Hugo A. Méndez-Hernández, Rosa M. Galaz-Ávalos, Víctor M. Loyola-Vargas
      Pages 179-188

    6. Somatic Embryogenesis in Common BeanPhaseolus vulgaris L.

      • José Luis Cabrera-Ponce, Itzel Anayetzi González-Gómez, Claudia G. León-Ramírez, José A. Sánchez-Arreguín, Alba E. Jofre y Garfias
      Pages 189-206

    7. Induction of Somatic Embryogenesis in Jatropha curcas

      • Rosa M. Galaz-Ávalos, Heydi G. Martínez-Sánchez, Víctor M. Loyola-Vargas
      Pages 207-214

    8. In Vitro Proliferation of Female Buds for Induction of Somatic Embryogenesis from False Horn Plantain (AAB, cv. Curraré)

      • Rosa Maria Escobedo-Gracia-Medrano, Carlos Iván Cruz-Cárdenas, Lucila Aurelia Sánchez-Cach, José Roberto Ku-Cauich, Wilma Aracely González-Kantún
      Pages 215-226

    9. Somatic Embryogenesis in Theobroma cacao L.

      • Claudia Garcia, Jean-Philippe Marelli, Juan Carlos Motamayor, Cristiano Villela
      Pages 227-245

    10. Somatic Embryogenesis of Quercus suber L. From Immature Zygotic Embryos

      • Pilar S. Testillano, Aránzazu Gómez-Garay, Beatriz Pintos, María C. Risueño
      Pages 247-256
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Keywords

About this book

This fourth edition provides new and updated protocols on plant cell, tissue, and organ cultures. Chapters are divided into five parts that cover topics from general methodologies, statistical analysis and contamination control, highly specialized techniques, and laborious process of measuring the epigenetics changes in tissue cultures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Plant Cell Culture Protocols, Fourth Edition aims to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Editors and Affiliations

  • Centro de Investigación Científica de Yucatán, Unidad de Bioquímica y Biología, Molecular de Plantas, Mérida, Mexico

    Víctor M. Loyola-Vargas

  • Departamento de Ingeniería Genética, Unidad Irapuato, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Irapuato, Mexico

    Neftalí Ochoa-Alejo

Bibliographic Information

  • Book Title: Plant Cell Culture Protocols

  • Editors: Víctor M. Loyola-Vargas, Neftalí Ochoa-Alejo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8594-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8593-7Published: 07 July 2018

  • Softcover ISBN: 978-1-4939-9337-6Published: 27 September 2019

  • eBook ISBN: 978-1-4939-8594-4Published: 06 July 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 4

  • Number of Pages: XV, 507

  • Number of Illustrations: 11 b/w illustrations, 90 illustrations in colour

  • Topics: Plant Sciences

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