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Nanoscale Imaging

Methods and Protocols

  • Book
  • © 2018
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Overview

Editors:
  1. Yuri L. Lyubchenko

    1. Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1814)

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Table of contents (34 protocols)

  1. Front Matter

    Pages i-xix
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  2. Imaging and Probing of Biomolecules

    1. Front Matter

      Pages 1-1
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    2. High-Resolution Atomic Force Microscopy Imaging of Nucleic Acids

      • Pablo Ares, Julio Gomez-Herrero, Fernando Moreno-Herrero
      Pages 3-17
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    3. Two Ligand Binding Sites in Serotonin Transporter Revealed by Nanopharmacological Force Sensing

      • Rong Zhu, Hermann J. Gruber, Peter Hinterdorfer
      Pages 19-33
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    4. AFM-Based Single-Molecule Force Spectroscopy of Proteins

      • Zackary N. Scholl, Piotr E. Marszalek
      Pages 35-47
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    5. High-Resolution AFM-Based Force Spectroscopy

      • Krishna P. Sigdel, Anna E. Pittman, Tina R. Matin, Gavin M. King
      Pages 49-62
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    6. Polymer Nanoarray Approach for the Characterization of Biomolecular Interactions

      • Sibaprasad Maity, Ekaterina Viazovkina, Alexander Gall, Yuri L. Lyubchenko
      Pages 63-74
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    7. Measuring Single-Molecule Twist and Torque in Multiplexed Magnetic Tweezers

      • Franziska Kriegel, Willem Vanderlinden, Thomas Nicolaus, Angelika Kardinal, Jan Lipfert
      Pages 75-98
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    8. AFM-Based Characterization of Electrical Properties of Materials

      • John Alexander, Sergey Belikov, Sergei Magonov
      Pages 99-127
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    9. Supported Lipid Bilayers for Atomic Force Microscopy Studies

      • Zhengjian Lv, Siddhartha Banerjee, Karen Zagorski, Yuri L. Lyubchenko
      Pages 129-143
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    10. Quantifying Small Molecule Binding Interactions with DNA Nanostructures

      • Xuye Lang, Yingning Gao, Ian Wheeldon
      Pages 145-155
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  3. High-Speed AFM of Biomolecules

    1. Front Matter

      Pages 157-157
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    2. Optimum Substrates for Imaging Biological Molecules with High-Speed Atomic Force Microscopy

      • Takayuki Uchihashi, Hiroki Watanabe, Noriyuki Kodera
      Pages 159-179
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    3. High-Resolution and High-Speed Atomic Force Microscope Imaging

      • Francesca Zuttion, Lorena Redondo-Morata, Arin Marchesi, Ignacio Casuso
      Pages 181-200
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    4. High-Speed Atomic Force Microscopy of Individual Amyloidogenic Protein Assemblies

      • Takahiro Watanabe-Nakayama, Kenjiro Ono
      Pages 201-212
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    5. Direct Observation of Dynamic Movement of DNA Molecules in DNA Origami Imaged Using High-Speed AFM

      • Masayuki Endo, Hiroshi Sugiyama
      Pages 213-224
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    6. Assembly of Centromere Chromatin for Characterization by High-Speed Time-Lapse Atomic Force Microscopy

      • Micah P. Stumme-Diers, Siddhartha Banerjee, Zhiqiang Sun, Yuri L. Lyubchenko
      Pages 225-242
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    7. High-Speed Force Spectroscopy for Single Protein Unfolding

      • Fidan Sumbul, Arin Marchesi, Hirohide Takahashi, Simon Scheuring, Felix Rico
      Pages 243-264
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  4. Imaging and Probing of Biomolecular Complexes

    1. Front Matter

      Pages 265-265
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    2. Probing RNA–Protein Interactions with Single-Molecule Pull-Down Assays

      • Mohamed Fareh, Chirlmin Joo
      Pages 267-285
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Keywords

About this book

This volume presents readers with the latest techniques to study nanoimaging and nanoprobing in application to a broad range of biological systems. The chapters in this book are divided into five parts, and cover topics such as imaging and probing of biomacromolecules including high-speed imaging and probing with AFM; probing chromatin structure with magnetic tweezers; and fluorescence correlation spectroscopy on genomic DNA in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and through, Nanoscale Imaging: Methods and Protocols is a valuable resource for anyone interested in learning more about this developing and expanding field.


Editors and Affiliations

  • Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, USA

    Yuri L. Lyubchenko

Bibliographic Information

  • Book Title: Nanoscale Imaging

  • Book Subtitle: Methods and Protocols

  • Editors: Yuri L. Lyubchenko

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8591-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8590-6Published: 29 June 2018

  • Softcover ISBN: 978-1-4939-9336-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8591-3Published: 28 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIX, 600

  • Number of Illustrations: 6 b/w illustrations, 170 illustrations in colour

  • Topics: Imaging / Radiology

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