Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1813)
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Table of contents (28 protocols)
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Front Matter
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Introduction
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Front Matter
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ADP-Ribose Binding Domains as Tools
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Front Matter
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Enzymatic Assays of NAD+ Utilizing Enzymes
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Front Matter
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Small Molecule Screening Assays of NAD+ Utilizing Enzymes
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Front Matter
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About this book
This volume focuses on mono-ADP-ribosylation and enzymes that use NAD+ including Sirtuins, PARPs, and bacterial and eukaryotic ADP-ribosyltransferases. The chapters in this book are organized into eight parts, and offer detailed descriptions of key protocols used to study topics such as in vitro techniques for ADP-ribosylation substrate identification; biochemical and biophysical assays of PAR-WWE domain interactions; monitoring expression and enzyme activity of ecto-ARTCs; HPLC-based enzymes assays for Sirtuins; and identifying target RNAs of PARPs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, ADP-ribosylation and NAD+ Utilizing Enzymes: Methods and Protocols is a valuable resource for anyone interested in this developing and expanding field.
Editors and Affiliations
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Department of Biology, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, USA
Paul Chang
Bibliographic Information
Book Title: ADP-ribosylation and NAD+ Utilizing Enzymes
Book Subtitle: Methods and Protocols
Editors: Paul Chang
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-8588-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018
Hardcover ISBN: 978-1-4939-8587-6Published: 11 August 2018
Softcover ISBN: 978-1-4939-9335-2Published: 10 December 2019
eBook ISBN: 978-1-4939-8588-3Published: 10 August 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 417
Number of Illustrations: 44 b/w illustrations, 51 illustrations in colour
Topics: Biochemistry, general