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DNA Nanotechnology

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Giampaolo Zuccheri

    1. Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1811)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. DNA Nanotechnology: From the Pub to Information-Based Chemistry

    • Nadrian C. Seeman
    Pages 1-9

  3. The Synthesis of Designer DNA

    • Rachel C. Little, Colette J. Whitfield, Eimer M. Tuite, Andrew R. Pike
    Pages 11-21

  4. Synthesis of DNA-Based Nanowires

    • Alexander Kotlyar
    Pages 23-47

  5. Preparation of Anti-miR PNAs for Drug Development and Nanomedicine

    • Alex Manicardi, Roberto Gambari, Luisa de Cola, Roberto Corradini
    Pages 49-63

  6. Loading of PNA and Other Molecular Payloads on Inorganic Nanostructures for Theranostics

    • Alessandro Bertucci, Simone Silvestrini, Roberto Corradini, Luisa De Cola
    Pages 65-77

  7. Design and Characterization of pH-Triggered DNA Nanoswitches and Nanodevices Based on DNA Triplex Structures

    • Andrea Idili, Francesco Ricci
    Pages 79-100

  8. Characterizing Surface-Immobilized DNA Structures and Devices Using a Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D)

    • Katherine E. Dunn, Martin A. Trefzer, Steven Johnson, Andy M. Tyrrell
    Pages 101-114

  9. Hybridization Chain Reaction Design and Biosensor Implementation

    • Andrea Miti, Giampaolo Zuccheri
    Pages 115-135

  10. Ultrasensitive Detection of Metal Ions with DNA Nanostructure

    • Mingshu Xiao, Xiangmeng Qu, Li Li, Hao Pei
    Pages 137-149

  11. Spatially Resolved Peptide-DNA Nanoassemblages for Biomarker Detection: A Synergy of DNA-Directed Immobilization and Nanografting

    • Abimbola Feyisara Adedeji, Elena Ambrosetti, Loredana Casalis, Matteo Castronovo
    Pages 151-162

  12. LSPR Detection of Nucleic Acids on Nanoparticle Monolayers

    • Sophie Thamm, Andrea Csàki, Wolfgang Fritzsche
    Pages 163-171

  13. DNA-Templated Silver Nanoclusters for DNA Methylation Detection

    • Hanie Ahmadzade Kermani, Morteza Hosseini, Mehdi Dadmehr
    Pages 173-182

  14. Selection of Structure-Switching DNA Aptamers Binding Soluble Small Molecules and SPR Validation of Enrichment

    • Enrico Tenaglia, Fabio M. Spiga, Carlotta Guiducci
    Pages 183-197

  15. Dielectrophoretic Stretching of DNA

    • Eva-Maria Laux, Frank F. Bier, Ralph Hölzel
    Pages 199-208

  16. A Practical Guide to Molecular Dynamics Simulations of DNA Origami Systems

    • Jejoong Yoo, Chen-Yu Li, Scott Michael Slone, Christopher Maffeo, Aleksei Aksimentiev
    Pages 209-229

  17. Single-Molecule Patterning via DNA Nanostructure Assembly: A Reusable Platform

    • Da Huang, Mark Freeley, Matteo Palma
    Pages 231-251

  18. Directed Protein Adsorption Through DNA Origami Masks

    • Saminathan Ramakrishnan, Guido Grundmeier, Adrian Keller
    Pages 253-262

  19. DNA Origami Structures Interfaced to Inorganic Nanodevices

    • Claudia Dalmastri, Weihua Han, Stefano Vespucci, Liqian Wang, Piero Morales
    Pages 263-278

  20. Tuning Gold Nanoparticles Plasmonic Properties by DNA Nanotechnology

    • Valentina Masciotti, Denys Naumenko, Marco Lazzarino, Luca Piantanida
    Pages 279-297
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Keywords

About this book

This second edition provides new and updated methods that detail new DNA nanotechnology techniques. Chapters focus on DNA origami nanostructures for arranging matter in the nanoscale or on their manipulation with the aid of other technologies, on procedures for making nucleic acids nanostructures of different kinds, and methods to simulate complex nanostructures or to use them in biosensing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and cutting-edge, DNA Nanotechnology: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Pharmacy and Biotechnology, University of Bologna, Bologna, Italy

    Giampaolo Zuccheri

Bibliographic Information

  • Book Title: DNA Nanotechnology

  • Book Subtitle: Methods and Protocols

  • Editors: Giampaolo Zuccheri

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8582-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8581-4Published: 21 June 2018

  • Softcover ISBN: 978-1-4939-9333-8Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8582-1Published: 20 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 334

  • Number of Illustrations: 33 b/w illustrations, 74 illustrations in colour

  • Topics: Biotechnology

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