Skip to main content
  • Book
  • © 2018

Handbook of ELISPOT

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1808)

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (20 protocols)

  1. Front Matter

    Pages i-x
  2. Essential Controls for ELISpot Assay

    • Jodi Hagen, Alexander E. Kalyuzhny
    Pages 31-41
  3. Automatic Search of Spots and Color Classification in ELISPOT Assay

    • Sergey S. Zadorozhny, Nikolai N. Martynov
    Pages 43-50
  4. Four Color ImmunoSpot® Assays for Identification of Effector T-Cell Lineages

    • Jodi Hanson, Diana R. Roen, Paul V. Lehmann
    Pages 51-62
  5. Detection of Cross-Reactive B Cells Using the FluoroSpot Assay

    • Peter Jahnmatz, Niklas Ahlborg
    Pages 63-72
  6. Multiplex ImmunoSpot® Assays for the Study of Functional B Cell Subpopulations

    • Diana R. Roen, Jodi Hanson, Paul V. Lehmann
    Pages 73-83
  7. Multi-Color FLUOROSPOT Counting Using ImmunoSpot® Fluoro-X™ Suite

    • Zoltán Megyesi, Paul V. Lehmann, Alexey Y. Karulin
    Pages 115-131
  8. Identification of Novel Mycobacterial Targets for Murine CD4+ T-Cells by IFNγ ELISPOT

    • Alison J. Johnson, Steven C. Kennedy, Tony W. Ng, Steven A. Porcelli
    Pages 143-150
  9. Cultured ELISpot Assay to Investigate Dengue Virus Specific T-Cell Responses

    • Chandima Jeewandara, Graham S. Ogg, Gathsaurie Neelika Malavige
    Pages 165-171
  10. Ex Vivo ELISpot Assay to Investigate iNKT Cell Responses in Acute Dengue Infection

    • Achala Indika Kamaladasa, Gathsaurie Neelika Malavige
    Pages 181-186
  11. Utilization of Feline ELISpot to Evaluate the Immunogenicity of a T Cell-Based FIV MAP Vaccine

    • Bikash Sahay, Alek M. Aranyos, Andrew McAvoy, Janet K. Yamamoto
    Pages 197-219
  12. Detection and Quantification of Influenza A/H1N1 Virus-Specific Memory B Cells in Human PBMCs Using ELISpot Assay

    • Iana H. Haralambieva, Inna G. Ovsyannikova, Richard B. Kennedy, Gregory A. Poland
    Pages 221-236

About this book

This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue virus, and tuberculosis; and vaccine research, essentials control, and assay evaluation in ELISpot. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Handbook of ELISPOT: Methods and Protocols, Third Edition is a valuable resource for both novice researchers and experienced ELISpot users.”

Editors and Affiliations

  • Bio-Techne, Inc., Minneapolis, USA

    Alexander E. Kalyuzhny

Bibliographic Information

  • Book Title: Handbook of ELISPOT

  • Book Subtitle: Methods and Protocols

  • Editors: Alexander E. Kalyuzhny

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8567-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8566-1Published: 29 June 2018

  • Softcover ISBN: 978-1-4939-9328-4Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8567-8Published: 28 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: X, 251

  • Number of Illustrations: 6 b/w illustrations, 68 illustrations in colour

  • Topics: Immunology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access