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Gangliosides

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Sandro Sonnino

    1. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Italy

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    You can also search for this editor in PubMed   Google Scholar

  2. Alessandro Prinetti

    1. Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Italy

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of gangliosides
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1804)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xv
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  2. Chemical and Physicochemical Properties of Gangliosides

    • Laura Mauri, Sandro Sonnino, Alessandro Prinetti
    Pages 1-17

  3. Gangliosides of the Nervous System

    • Robert Ledeen, Gusheng Wu
    Pages 19-55

  4. Scattering Techniques and Ganglioside Aggregates: Laser Light, Neutron, and X-Ray Scattering

    • Elena Del Favero, Paola Brocca, Laura Cantù
    Pages 57-82

  5. Gangliosides in the Immune System: Role of Glycosphingolipids and Glycosphingolipid-Enriched Lipid Rafts in Immunological Functions

    • Kazuhisa Iwabuchi
    Pages 83-95

  6. Ganglioside Metabolism and Its Inherited Diseases

    • Bernadette Breiden, Konrad Sandhoff
    Pages 97-141

  7. Gangliosides and Tumors

    • Reiji Kannagi, Bi-He Cai, Hsiang-Chi Huang, Chia-Chun Chao, Keiichiro Sakuma
    Pages 143-171

  8. Anti-ganglioside Antibodies in Peripheral Nerve Pathology

    • Hugh J. Willison
    Pages 173-188

  9. Protocols for Glycosyltransferase Assays: Ganglioside Globoside and Lewis-X Intermediate-Lactosylceramide Biosyntheses in Eukaryotic Systems

    • Subhash Basu, Arun Agarwal, Manju Basu, Rui Ma, Joseph R. Moskal
    Pages 189-205

  10. Mass Spectrometry of Gangliosides

    • Akemi Suzuki, Minoru Suzuki, Emi Ito, Takahiro Nitta, Jin-ichi Inokuchi
    Pages 207-221

  11. Visualization of Brain Gangliosides Using MALDI Imaging Mass Spectrometry

    • Eiji Sugiyama, Mitsutoshi Setou
    Pages 223-229

  12. Immunoelectron Microscopy of Gangliosides

    • Takuma Tsuji, Akikazu Fujita, Toyoshi Fujimoto
    Pages 231-239

  13. Nuclear Magnetic Resonance of Gangliosides

    • Domenico Acquotti, Laura Mauri, Sandro Sonnino
    Pages 241-284

  14. Methods for the Preparation of Anti-ganglioside Monoclonal Antibodies

    • Noriyuki Yuasa, Ikuo Kawashima
    Pages 285-291

  15. Chemical Synthesis of Gangliosides

    • Akihiro Imamura, Makoto Kiso
    Pages 293-310

  16. Radioactive Gangliosides for Biological Studies

    • Laura Mauri, Simona Prioni, Maria Grazia Ciampa, Sandro Sonnino
    Pages 311-322

  17. Synthesis of Fluorescent Gangliosides

    • Günter Schwarzmann
    Pages 323-356

  18. Synthesis of Photoactivatable and Paramagnetic Gangliosides

    • Günter Schwarzmann
    Pages 357-381

  19. Methods for Assay of Ganglioside Catabolic Enzymes

    • Nicoletta Loberto, Giulia Lunghi, Domitilla Schiumarini, Maura Samarani, Elena Chiricozzi, Massimo Aureli
    Pages 383-400

  20. Pharmacological Modulation of Glycosphingolipid Metabolism

    • Jin-ichi Inokuchi, Takashi Ode, Miki Hara-Yokoyama
    Pages 401-410
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Keywords

About this book

This detailed volume explores methods of examining gangliosides, membrane amphiphilic lipids that are deeply involved in the modulation of the overall properties of cellular membranes, as well as of the functions of membrane-associated proteins. The contents take into account the vital availability of new enzymatic approaches, the knowledge of an incredibly extensive number of glyco-enzymes, and the improvement of multi-genetic analyses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Gangliosides: Methods and Protocols serves as a valuable guidebook for entering in the vast research world of gangliosides.

Editors and Affiliations

  • Department of Medical Biotech & Translational Medicine, University of Milan, Segrate, Italy

    Sandro Sonnino, Alessandro Prinetti

Bibliographic Information

  • Book Title: Gangliosides

  • Book Subtitle: Methods and Protocols

  • Editors: Sandro Sonnino, Alessandro Prinetti

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8552-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8551-7Published: 21 June 2018

  • Softcover ISBN: 978-1-4939-9323-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-8552-4Published: 20 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 456

  • Number of Illustrations: 45 b/w illustrations, 56 illustrations in colour

  • Topics: Biochemistry, general

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