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Immunotoxicity Testing

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Jamie C. DeWitt

    1. Department of Pharmacology and Toxicology, East Carolina University, Greenville, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Cheryl E. Rockwell

    1. Department of Pharmacology and Toxicology, Michigan State University, East Lansing, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Christal C. Bowman

    1. Durham, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of immunotoxicity testing
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1803)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Overview and Health Risk Considerations

    1. Front Matter

      Pages 1-1
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    2. Immunotoxicology: A Brief History

      • Kenneth L. Hastings
      Pages 3-13

    3. Clinical Immunotoxicology

      • L. Peyton Myers
      Pages 15-26

    4. Investigative Immunotoxicology

      • Stacey E. Anderson, Hillary L. Shane
      Pages 27-46

    5. Developmental Immunotoxicity (DIT) Testing: Current Recommendations and the Future of DIT Testing

      • Annalise N. vonderEmbse, Jamie C. DeWitt
      Pages 47-56

    6. Markers of Inflammation

      • Dori R. Germolec, Kelly A. Shipkowski, Rachel P. Frawley, Ellen Evans
      Pages 57-79

  3. Legacy Protocols: In Vivo

    1. Front Matter

      Pages 81-81
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    2. The Sheep Erythrocyte T-Dependent Antibody Response (TDAR)

      • Gregory S. Ladics
      Pages 83-94

    3. Methylated Bovine Serum Albumin (mBSA)-Induced Delayed-Type Hypersensitivity in Mice

      • Jessica Margaret Sido
      Pages 95-99

    4. Use of the LLNA:BrdU-ELISA for Skin Sensitization Hazard Assessment

      • David M. Lehmann
      Pages 101-116

    5. Host Resistance Assays

      • Stefanie C. M. Burleson, Wendy Jo Freebern, Florence G. Burleson, Gary R. Burleson, Victor J. Johnson, Robert W. Luebke
      Pages 117-145

    6. Enhanced Histopathology Evaluation of Lymphoid Organs

      • Susan A. Elmore
      Pages 147-168

    7. Tumor Challenges in Immunotoxicity Testing

      • Sheung P. Ng, Judith T. Zelikoff
      Pages 169-180

  4. Legacy Protocols: Ex Vivo and In Vitro

    1. Front Matter

      Pages 181-181
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    2. Flow Cytometry for the Immunotoxicologist

      • Scott T. Espenschied, Robert M. Tighe, Kymberly M. Gowdy
      Pages 183-197

    3. Evaluation of Cell-Mediated Immune Function Using the Cytotoxic T-Lymphocyte Assay

      • Gary R. Burleson, Florence G. Burleson, Rodney R. Dietert
      Pages 199-208

    4. Evaluation of Cell Proliferation and Apoptosis in Immunotoxicity Testing

      • Mitzi Nagarkatti, Sadiye Amcaoglu Rieder, Prakash S. Nagarkatti
      Pages 209-230

    5. Natural Killer (NK) Cell Assays in Immunotoxicity Testing

      • Qing Li
      Pages 231-241

    6. Dendritic Cell Assays

      • Joanna M. Kreitinger, David M. Shepherd
      Pages 243-253
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Keywords

About this book

This fully updated volume utilizes the expertise of scientists currently engaged in immunotoxicity testing to provide the reader with lab-ready procedures and the background information needed to identify effective testing approaches. Dedicated to identifying and describing exogenous agents that can modify immune function, uncovering modes and mechanisms of action for such agents, and translating data from the laboratory and from the clinic to better predict health risks as well as benefits to those who are exposed to immunomodulatory agents, immunotoxicity testing continues to be a vital field of study, and this collection highlights both the “tried and true” methods as well as alternative protocols that have been more recently developed.  Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, andtips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Immunotoxicity Testing: Methods and Protocols, Second Edition serves as a valuable contribution to the continued evolution and the application of immunotoxicity testing.

Editors and Affiliations

  • Department of Pharmacology and Toxicology, East Carolina University, Greenville, USA

    Jamie C. DeWitt

  • Department of Pharmacology and Toxicology, Michigan State University, East Lansing, USA

    Cheryl E. Rockwell

  • Durham, USA

    Christal C. Bowman

Bibliographic Information

  • Book Title: Immunotoxicity Testing

  • Book Subtitle: Methods and Protocols

  • Editors: Jamie C. DeWitt, Cheryl E. Rockwell, Christal C. Bowman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-8549-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-8548-7Published: 08 June 2018

  • Softcover ISBN: 978-1-4939-9322-2Published: 25 July 2019

  • eBook ISBN: 978-1-4939-8549-4Published: 07 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 412

  • Number of Illustrations: 18 b/w illustrations, 23 illustrations in colour

  • Topics: Pharmacology/Toxicology

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