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Multiple Myeloma

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Christoph Heuck

    1. Janssen R&D, Spring House, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Niels Weinhold

    1. The Myeloma Institute, University of Arkansas for Medical Science, Little Rock, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides a step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1792)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. ELDA qASO-PCR for High Sensitivity Detection of Tumor Cells in Bone Marrow and Peripheral Blood

    • Stefanie Huhn
    Pages 1-14

  3. Retracted Chapter: EuroFlow-Based Next-Generation Flow Cytometry for Detection of Circulating Tumor Cells and Minimal Residual Disease in Multiple Myeloma

    • Leire Burgos, Bruno Paiva
    Pages 15-34

  4. Cytoplasmic Immunoglobulin Vs. DNA Analysis by Flow Cytometry

    • Daisy Alapat
    Pages 35-45

  5. Deep Profiling of the Immune System of Multiple Myeloma Patients Using Cytometry by Time-of-Flight (CyTOF)

    • Tina Smets, Frederik Stevenaert, HC Adams 3rd, Greet Vanhoof
    Pages 47-54

  6. Fluorescence In Situ Hybridization (FISH) in Multiple Myeloma

    • Erming Tian
    Pages 55-69

  7. Whole Exome Sequencing in Multiple Myeloma to Identify Somatic Single Nucleotide Variants and Key Translocations Involving Immunoglobulin Loci and MYC

    • Brian A. Walker
    Pages 71-95

  8. RNA-Sequencing from Low-Input Material in Multiple Myeloma for Application in Clinical Routine

    • Anja Seckinger, Tomi Bähr-Ivacevic, Vladimir Benes, Dirk Hose
    Pages 97-115

  9. Protocol for M3P: A Comprehensive and Clinical Oriented Targeted Sequencing Panel for Routine Molecular Analysis in Multiple Myeloma

    • Santiago Barrio, Matteo DáVia, Laura Bruins, Thorsten Stühmer, Torsten Steinbrunn, Max Bittrich et al.
    Pages 117-128

  10. Analysis of Circulating Tumor DNA

    • Sridurga Mithraprabhu, Andrew Spencer
    Pages 129-145

  11. Detection of Cross-Sample Contamination in Multiple Myeloma Samples and Sequencing Data

    • Owen W. Stephens, Tobias Meißner, Niels Weinhold
    Pages 147-155

  12. Analysis of Global Gene Expression Profiles

    • Alboukadel Kassambara, Jerome Moreaux
    Pages 157-166

  13. Genome Wide Mapping of Methylated and Hydroxyl-Methylated Cytosines Using a Modified HpaII Tiny Fragment Enrichment by Ligation Mediated PCR Tagged Sequencing Protocol

    • Sanchari Battachariyya, Ruslana Tytarenko, Christoph Heuck, John Greally, Amit Verma
    Pages 167-177

  14. A Rapid and Robust Protocol for Reduced Representation Bisulfite Sequencing in Multiple Myeloma

    • Samrat Roy Choudhury, Brian A. Walker
    Pages 179-191

  15. Microfluidic Production and Application of Lipid Nanoparticles for Nucleic Acid Transfection

    • Anitha Thomas, Shyam M. Garg, Rebecca A. G. De Souza, Eric Ouellet, Grace Tharmarajah, Dave Reichert et al.
    Pages 193-203

  16. Microfluidic Assembly of Liposomes with Tunable Size and Coloading Capabilities

    • Jessica R. Hoffman, Ennio Tasciotti, Roberto Molinaro
    Pages 205-214

  17. Retraction Note to: EuroFlow-Based Next-Generation Flow Cytometry for Detection of Circulating Tumor Cells and Minimal Residual Disease in Multiple Myeloma

    • Leire Burgos, Bruno Paiva
    Pages E1-E1

  18. Correction to: Multiple Myeloma

    • Christoph Heuck, Niels Weinhold
    Pages E3-E3

  19. Back Matter

    Pages 215-216
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Keywords

About this book

This volume provides a thorough guide of the study of multiple myeloma (MM)—from sample acquisition to the performance of molecular tests, and also discusses difficulties that arise during quality control and interpretation of results. The chapters in this book cover topics, such as next-generation-flow-based methods for detection of minimal residual disease and circulating tumor cells; Cytometry by Time-of-Flights (CyTOF); a droplet digital PCR approach for finding contamination in patients’ samples; genome-wide investigation of the methylation and hydroxymethylation status of cytosines; and a microfluidic approach for creating nanoparticles. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Multiple Myeloma: Methods and Protocols is a valuable resource for molecular scientists and laboratory supervisors working with MM samples, and any investigator conducting studies in this field.



Editors and Affiliations

  • Janssen R&D, Spring House, USA

    Christoph Heuck

  • The Myeloma Institute, University of Arkansas for Medical Science, Little Rock, USA

    Niels Weinhold

Bibliographic Information

  • Book Title: Multiple Myeloma

  • Book Subtitle: Methods and Protocols

  • Editors: Christoph Heuck, Niels Weinhold

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7865-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7864-9Published: 25 May 2018

  • Softcover ISBN: 978-1-4939-9308-6Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7865-6Published: 24 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 216

  • Number of Illustrations: 9 b/w illustrations, 32 illustrations in colour

  • Topics: Cancer Research

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