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  • © 2018

Reporter Gene Imaging

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1790)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Optical Bioluminescence Protocol for Imaging Mice

    • David Stout, John David
    Pages 29-40
  3. Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase

    • Anne E. Gibbons, Kathryn E. Luker, Gary D. Luker
    Pages 41-50
  4. Reporter-Based BRET Sensors for Measuring Biological Functions In Vivo

    • Maitreyi Rathod, Arijit Mal, Abhijit De
    Pages 51-74
  5. In Vivo Bacterial Imaging Using Bioluminescence

    • Mariette Barbier, Justin Bevere, F. Heath Damron
    Pages 87-97
  6. Hypoxia-Induced Reporter Genes with Different Half-Lives

    • Balaji Krishnamachary, Pierre Danhier, Samata Kakkad, Santosh K. Bharti, Zaver M. Bhujwalla
    Pages 113-125
  7. Ex Vivo Radiolabeling and In Vivo PET Imaging of T Cells Expressing Nuclear Reporter Genes

    • Maxim A. Moroz, Pat Zanzonico, Jason T. Lee, Vladimir Ponomarev
    Pages 153-163
  8. Imaging T Cell Dynamics and Function Using PET and Human Nuclear Reporter Genes

    • Jason T. Lee, Maxim A. Moroz, Vladimir Ponomarev
    Pages 165-180
  9. Practical Guidelines for Cerenkov Luminescence Imaging with Clinically Relevant Isotopes

    • Nikunj B. Bhatt, Darpan N. Pandya, William A. Dezarn, Frank C. Marini, Dawen Zhao, William H. Gmeiner et al.
    Pages 197-208
  10. Back Matter

    Pages 219-221

About this book

This volume provides an introduction for the scientist who is new to the field of molecular imaging, as well as detailed methods for experts in other areas of molecular imaging. Chapters detail the advantages and limitations of combining fluorescent, bioluminescent and radioisotopic, creating dual modality imaging reporter gene construct, bioluminescent imaging, and  bioluminescent or fluorescent imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Reporter Gene Imaging : Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Department of Microbial Infection and Immunity, The Ohio State University, Columbus, USA

    Purnima Dubey

Bibliographic Information

  • Book Title: Reporter Gene Imaging

  • Book Subtitle: Methods and Protocols

  • Editors: Purnima Dubey

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7860-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7858-8Published: 02 June 2018

  • Softcover ISBN: 978-1-4939-9306-2Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7860-1Published: 01 June 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 221

  • Number of Illustrations: 11 b/w illustrations, 42 illustrations in colour

  • Topics: Imaging / Radiology

Buy it now

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 99.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 139.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access