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Phenotypic Screening

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Bridget Wagner

    1. Chemical Biology and Therapeutics Science Program, The Broad Institute of MIT and Harvard, Cambridge, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1787)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Nutrient Stress Small-Molecule Screening Platform for Escherichia coli

    • Sara S. El Zahed, Garima Kumar, Madeline Tong, Eric D. Brown
    Pages 1-18

  3. Validation and Development of an Escherichia coli Riboflavin Pathway Phenotypic Screen Hit as a Small-Molecule Ligand of the Flavin Mononucleotide Riboswitch

    • Carl J. Balibar, Artjohn Villafania, Christopher M. Barbieri, Nick Murgolo, Terry Roemer, Hao Wang et al.
    Pages 19-40

  4. Phenotypic Screening of Small Molecules with Antimalarial Activity for Three Different Parasitic Life Stages

    • Nobutaka Kato, Sandra March, Sangeeta N. Bhatia, Matthias Marti
    Pages 41-52

  5. Phenotypic Screening for Inhibitors of a Mutant Thrombopoietin Receptor

    • Anna Ngo, Ann Koay, Christian Pecquet, Carmen C. Diaconu, David A. Jenkins, Andrew K. Shiau et al.
    Pages 53-66

  6. A Cell-Based Assay for Mitotic Spindle Orientation

    • Elina Glaubke, Holger Bastians
    Pages 67-75

  7. Isolation of Skeletal Muscle Stem Cells for Phenotypic Screens for Modulators of Proliferation

    • Aaron C. Hinken, Andrew N. Billin
    Pages 77-86

  8. Cell-Based Methods to Identify Inducers of Human Pancreatic Beta-Cell Proliferation

    • Courtney A. Ackeifi, Ethan A. Swartz, Peng Wang
    Pages 87-100

  9. High-Content Imaging Phenotypic Screen for Neurogenesis Using Primary Neural Progenitor Cells

    • Li Sharon Wu, Jingjun Li
    Pages 101-113

  10. Discovery of Modulators of Adipocyte Physiology Using Fully Functionalized Fragments

    • Andrea Galmozzi, Christopher G. Parker, Bernard P. Kok, Benjamin F. Cravatt, Enrique Saez
    Pages 115-127

  11. The Ancient Genetic Networks of Obesity: Whole-Animal Automated Screening for Conserved Fat Regulators

    • Wenfan Ke, Anna Drangowska-Way, Daniel Katz, Karsten Siller, Eyleen J. O’Rourke
    Pages 129-146

  12. Modulation of Threat Response in Larval Zebrafish

    • Andrew J. Rennekamp
    Pages 147-159

  13. A PLA-iRoCS Pipeline for the Localization of Protein–Protein Interactions In Situ

    • Taras Pasternak, William Teale, Thorsten Falk, Benedetto Ruperti, Klaus Palme
    Pages 161-170

  14. High-Dimensional Profiling: The Theta Comparative Cell Scoring Method

    • Scott J. Warchal, John C. Dawson, Neil O. Carragher
    Pages 171-181

  15. Accessing the Open PHACTS Discovery Platform with Workflow Tools

    • Daniela Digles, Andrei Caracoti, Edgar Jacoby
    Pages 183-193

  16. Quantitative Prioritization of Tool Compounds for Phenotypic Screening

    • Yuan Wang, Jeremy L. Jenkins
    Pages 195-206

  17. A Quantitative Systems Pharmacology Approach to Infer Pathways Involved in Complex Disease Phenotypes

    • Mark E. Schurdak, Fen Pei, Timothy R. Lezon, Diane Carlisle, Robert Friedlander, D. Lansing Taylor et al.
    Pages 207-222

  18. Phenotype-Based High-Content Screening Using Fluorescent Chemical Bioprobes: Lipid Droplets and Glucose Uptake Quantification in Live Cells

    • Young-Hee Shin, Seung Bum Park
    Pages 223-234

  19. Developing High-Throughput Assays to Analyze and Screen Electrophysiological Phenotypes

    • Jen Q. Pan, David Baez-Nieto, Andrew Allen, Hao-Ran Wang, Jeffrey R. Cottrell
    Pages 235-252

  20. Testing Susceptibility of Patient-Derived Organoid Cultures to Therapies: Pharmacotyping

    • Richard A. Burkhart, Lindsey A. Baker, Hervé Tiriac
    Pages 253-261
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Keywords

About this book

This volume provides a better understanding of the advancements in phenotypic readouts and improved disease models that generate novel biological insights and recapitulate clinically relevant biology. Chapters focus on phenotypic screening, the use of human cell models, microscopic approaches, assays to measure fat accumulation in C. elegans, the threat response in zebrafish, and protein-protein interactions in plant growth and development. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Phenotypic Screening: Methods and Protocols aims to make phenotypic screening approaches more accessible to a wide array of researchers throughout the academic and biotech communities.

Editors and Affiliations

  • Chemical Biology and Therapeutics Science Program, The Broad Institute of MIT and Harvard, Cambridge, USA

    Bridget Wagner

Bibliographic Information

  • Book Title: Phenotypic Screening

  • Book Subtitle: Methods and Protocols

  • Editors: Bridget Wagner

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7847-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7846-5Published: 08 May 2018

  • Softcover ISBN: 978-1-4939-9303-1Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7847-2Published: 07 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 280

  • Number of Illustrations: 16 b/w illustrations, 36 illustrations in colour

  • Topics: Biochemistry, general

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