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Macrophages

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Germain Rousselet

    1. CEA/DRF/Jacob/iRCM/LRTS, INSERM U967, Fontenay aux Roses, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1784)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Culture of Human Monocyte-Derived Macrophages

    • Aoife Kelly, Aleksander M. Grabiec, Mark A. Travis
    Pages 1-11

  3. A Simple Multistep Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages

    • Chandrayana Mukherjee, Christine Hale, Subhankar Mukhopadhyay
    Pages 13-28Open Access

  4. Growing Murine Bone Marrow-Derived Macrophages

    • Anaïs Assouvie, Lisa P. Daley-Bauer, Germain Rousselet
    Pages 29-33

  5. Immortalization of Murine Bone Marrow-Derived Macrophages

    • Dominic De Nardo, Dhan V. Kalvakolanu, Eicke Latz
    Pages 35-49

  6. Isolation and Identification of Murine Serous Cavity Macrophages

    • Calum C. Bain, Stephen J. Jenkins
    Pages 51-67

  7. Isolation and Identification of Interstitial Macrophages from the Lungs Using Different Digestion Enzymes and Staining Strategies

    • Shaikh M. Atif, Sophie L. Gibbings, Claudia V. Jakubzick
    Pages 69-76

  8. Isolation and Phenotyping of Adult Mouse Microglial Cells

    • Kathleen Grabert, Barry W. McColl
    Pages 77-86Open Access

  9. Isolation and Phenotyping of Bone Marrow Macrophages

    • Marion Chalot
    Pages 87-92

  10. Isolation and Phenotyping of Intestinal Macrophages

    • Vanessa Petit
    Pages 93-98

  11. Phenotyping Tumor-Associated Macrophages

    • R. Ian Cumming, Yen-Rei A. Yu
    Pages 99-109

  12. Activating Murine Macrophages In Vitro

    • Elizabeth Dalby
    Pages 111-117

  13. Polarizing Macrophages In Vitro

    • Xuan Huang, Yong Li, Mingui Fu, Hong-Bo Xin
    Pages 119-126

  14. Viral Replication Assay in Bone Marrow-Derived Macrophages

    • Linda Roback, Lisa P. Daley-Bauer
    Pages 127-134

  15. Macrophage Bactericidal Assays

    • Mourad Aribi
    Pages 135-149

  16. Quantitative Phagocytosis Assays in Primary and Cultured Macrophages

    • Fernando Montaño, Sergio Grinstein, Roni Levin
    Pages 151-163

  17. Observing Frustrated Phagocytosis and Phagosome Formation and Closure Using Total Internal Reflection Fluorescence Microscopy (TIRFM)

    • Anna Mularski, Florence Marie-Anaïs, Julie Mazzolini, Florence Niedergang
    Pages 165-175

  18. Chromatin Immunoprecipitation in Macrophages

    • Germain Rousselet
    Pages 177-186

  19. Transfecting Macrophages

    • Andrea-Anneliese Keller, Marten B. Maeß, Michael Schnoor, Berith Scheiding, Stefan Lorkowski
    Pages 187-195

  20. In Vitro Migration Assays

    • Lewis Taylor, Carlota Recio, David R. Greaves, Asif J. Iqbal
    Pages 197-214
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Keywords

About this book

This volume discusses basic and advanced techniques to study macrophages and their unique properties. The chapters in this book cover numerous topics such as in vitro culture models for murine and human macrophages; isolation of resident macrophages from several tissues; functional analyses of macrophages; and transgenic models of macrophage depletion and macrophage targeting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and comprehensive, Macrophages: Methods and Protocols is a valuable resource for researchers who are interested in studying macrophages on an experimental level.


The chapters A Simple Multi-Step Protocol for Differentiating Human Induced Pluripotent Stem Cells into Functional Macrophages and Isolation and Phenotyping of Adult Mouse Microglial Cells are open access under a CC BY 4.0 license via link.springer.com.


Editors and Affiliations

  • CEA/DRF/Jacob/iRCM/LRTS, INSERM U967, Fontenay aux Roses, France

    Germain Rousselet

About the editor



Bibliographic Information

  • Book Title: Macrophages

  • Book Subtitle: Methods and Protocols

  • Editors: Germain Rousselet

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7837-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7836-6Published: 15 May 2018

  • Softcover ISBN: 978-1-4939-9300-0Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7837-3Published: 14 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 278

  • Number of Illustrations: 8 b/w illustrations, 35 illustrations in colour

  • Topics: Cell Biology

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