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Mitochondrial Bioenergetics

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Carlos M. Palmeira

    1. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. António J. Moreno

    1. Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1782)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Overview of Mitochondrial Bioenergetics

    • Vitor M. C. Madeira
    Pages 1-6

  3. Evaluation of Respiration with Clark-Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells

    • Ana M. Silva, Paulo J. Oliveira
    Pages 7-29

  4. High-Resolution FluoRespirometry and OXPHOS Protocols for Human Cells, Permeabilized Fibers from Small Biopsies of Muscle, and Isolated Mitochondria

    • Carolina Doerrier, Luiz F. Garcia-Souza, Gerhard Krumschnabel, Yvonne Wohlfarter, András T. Mészáros, Erich Gnaiger
    Pages 31-70

  5. High-Throughput Analysis of Mitochondrial Oxygen Consumption

    • James Hynes, Rachel L. Swiss, Yvonne Will
    Pages 71-87

  6. Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices

    • Ana Ledo, Rui M. Barbosa, João Laranjinha
    Pages 89-107

  7. Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria

    • João Soeiro Teodoro, Carlos Marques Palmeira, Anabela Pinto Rolo
    Pages 109-119

  8. Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells

    • David G. Nicholls
    Pages 121-135

  9. Comparison of Mitochondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production

    • Timea Komlódi, Ondrej Sobotka, Gerhard Krumschnabel, Nicole Bezuidenhout, Elisabeth Hiller, Carolina Doerrier et al.
    Pages 137-155

  10. Measurement of Proton Leak in Isolated Mitochondria

    • Charles Affourtit, Hoi-Shan Wong, Martin D. Brand
    Pages 157-170

  11. Imaging Mitochondrial Calcium Fluxes with Fluorescent Probes and Single- or Two-Photon Confocal Microscopy

    • Sean M. Davidson, Michael R. Duchen
    Pages 171-186

  12. Mitochondrial Permeability Transition Pore and Calcium Handling

    • Randi J. Parks, Elizabeth Murphy, Julia C. Liu
    Pages 187-196

  13. Redox Equivalents and Mitochondrial Bioenergetics

    • James R. Roede, Young-Mi Go, Dean P. Jones
    Pages 197-227

  14. Mitochondrial Bioenergetics by 13C–NMR Isotopomer Analysis

    • Rui A. Carvalho, Ivana Jarak
    Pages 229-247

  15. Computational Modeling of Mitochondrial Function from a Systems Biology Perspective

    • Sonia Cortassa, Steven J. Sollott, Miguel A. Aon
    Pages 249-265

  16. Monitoring the Mitochondrial Dynamics in Mammalian Cells

    • Luca Simula, Silvia Campello
    Pages 267-285

  17. Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mitochondria

    • Hoi-Shan Wong, Pierre-Axel Monternier, Adam L. Orr, Martin D. Brand
    Pages 287-299

  18. Plate-Based Measurement of Respiration by Isolated Mitochondria

    • Shona A. Mookerjee, Casey L. Quinlan, Hoi-Shan Wong, Pratiksha Dighe, Martin D. Brand
    Pages 301-313

  19. Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells

    • Shun-ichi Yamashita, Tomotake Kanki
    Pages 315-324

  20. The Importance of Calcium Ions for Determining Mitochondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mitochondria Isolated from Brown Adipose Tissue

    • Kieran J. Clarke, Richard K. Porter
    Pages 325-336
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Keywords

About this book

This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mitochondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS protocols in human cells, analysis of mitochondrial oxygen consumption, mitochondrial bioenergetics, and mitochondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Mitochondrial Bioenergetics: Methods and Protocols, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mitochondrial genetics industries.



Editors and Affiliations

  • Department of Life Sciences, University of Coimbra, Coimbra, Portugal

    Carlos M. Palmeira, António J. Moreno

Bibliographic Information

  • Book Title: Mitochondrial Bioenergetics

  • Book Subtitle: Methods and Protocols

  • Editors: Carlos M. Palmeira, António J. Moreno

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7831-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7830-4Published: 31 May 2018

  • Softcover ISBN: 978-1-4939-9298-0Published: 25 July 2019

  • eBook ISBN: 978-1-4939-7831-1Published: 30 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 422

  • Number of Illustrations: 48 b/w illustrations, 59 illustrations in colour

  • Topics: Cell Biology

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