Amyloid Proteins

1. Front Matter

   Pages i-xvii

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2. In Vitro Models and Assays

   1. Front Matter

      Pages 1-1

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   2. Preparation of Pure Populations of Amyloid β-Protein Oligomers of Defined Size

      • Eric Y. Hayden, Joseph L. Conovaloff, Ashley Mason, Gal Bitan, David B. Teplow

      Pages 3-12

   3. Preparation of a Well-Defined and Stable β-Barrel Pore-Forming Aβ42 Oligomer

      • Montserrat Serra-Batiste, Martí Ninot-Pedrosa, Eduard Puig, Sonia Ciudad, Margarida Gairí, Natàlia Carulla

      Pages 13-22

   4. Unveiling Brain Aβ Heterogeneity Through Targeted Proteomic Analysis

      • Agueda Rostagno, Thomas A. Neubert, Jorge Ghiso

      Pages 23-43

   5. Preparation of α-Synuclein Amyloid Assemblies for Toxicity Experiments

      • Serene W. Chen, Nunilo Cremades

      Pages 45-60

   6. Generation and Characterization of Stable α-Synuclein Oligomers

      • Leire Almandoz-Gil, Martin Ingelsson, Joakim Bergström

      Pages 61-71

   7. In Vitro Analysis of α-Synuclein Amyloid Formation and Cross-Reactivity

      • Istvan Horvath, Sandra Rocha, Pernilla Wittung-Stafshede

      Pages 73-83

   8. Preparation of Tau Oligomers After the Protein Extraction from Bacteria and Brain Cortices

      • Elentina K. Argyrousi, Agnieszka Staniszewski, Russell E. Nicholls, Ottavio Arancio

      Pages 85-97

   9. Purification and Characterization of Low-n Tau Oligomers

      • Senthilvelrajan Kaniyappan, Ram Reddy Chandupatla, Eckhard Mandelkow

      Pages 99-111

   10. Preparation and Characterization of Tau Oligomer Strains

       • Urmi Sengupta, Mariana Carretero-Murillo, Rakez Kayed

       Pages 113-146

   11. Purification and Fibrillation of Recombinant Human Amyloid-β, Prion Protein, and Tau Under Native Conditions

       • Alexander Sandberg, Sofie Nyström

       Pages 147-166

   12. Preparation of Amyloidogenic Aggregates from EF-Hand β-Parvalbumin and S100 Proteins

       • Javier Martínez, Joana S. Cristóvão, Rosa Sánchez, Maria Gasset, Cláudio M. Gomes

       Pages 167-179

   13. Kinetic Analysis of Amyloid Formation

       • Georg Meisl, Thomas C. T. Michaels, Sara Linse, Tuomas P. J. Knowles

       Pages 181-196

   14. Mapping Amyloid Regions in Gad m 1 with Peptide Arrays

       • Rosa Sánchez, Javier Martínez, Laura Montoya, Milagros Castellanos, Maria Gasset

       Pages 197-207

   15. Noninvasive Structural Analysis of Intermediate Species During Fibrillation: An Application of Small-Angle X-Ray Scattering

       • Annette Eva Langkilde, Fátima Herranz-Trillo, Pau Bernadó, Bente Vestergaard

       Pages 209-239

   16. Analysis of Covalent Modifications of Amyloidogenic Proteins Using Two-Dimensional Electrophoresis: Prion Protein and Its Sialylation

       • Elizaveta Katorcha, Ilia V. Baskakov

       Pages 241-255

   17. Amplification and Detection of Minuscule Amounts of Misfolded Prion Protein by Using the Real-Time Quaking-Induced Conversion

       • Matthias Schmitz, Niccolò Candelise, Franc Llorens, Inga Zerr

       Pages 257-263

3. Cell Culture Models and Assays

   1. Front Matter

      Pages 265-265

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   2. Bacterial Amyloids

      • Margery L. Evans, Elizabeth Gichana, Yizhou Zhou, Matthew R. Chapman

      Pages 267-288

This third edition volume expands on the previous editions with many new chapters that cover the latest techniques and topics that were not addressed in the previous volumes. The chapters in this book are divided into three parts: Part One covers in vitro assays that focus on a variety  of amyloids and how to study these peptides and proteins. Part Two describes cell culture models and assays, and Part Three explores methods on how to extract amyloid from tissue, its detection, and its characterization in vitro or in vivo. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and authoritative, Amyloid Proteins: Methods and Protocols, Third Edition is a valuable resource for both students and scientists who are new to the field, as well as experienced researchers who would like to progress their research with the latest available techniques.

• Fibrillogenesis
• Quartz Crystal
• Fibre Diffraction
• Fluorescence Microscopy
• Affinity Chromatography

• Department of Neuroscience and Physiology, New York University School of Medicine, New York University Langone Health, New York, USA

  Einar M. Sigurdsson

• Chronic Disease Programme-CROSADIS CIBERNED, Queen Sofia Foundation, Alzheimer Center CIEN Foundation, Instituto de Salud Carlos III, Madrid, Spain

  Miguel Calero

• Institute of Physical Chemistry “Rocasolano”, Spanish National Research Council (CSIC), Madrid, Spain

  María Gasset

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