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Peptide Self-Assembly

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Bradley L. Nilsson

    1. Department of Chemistry, University of Rochester, Rochester, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Todd M. Doran

    1. Department of Medicinal Chemistry, Institute for Translational Neuroscience, University of Minnesota, Minneapolis, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1777)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xv
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  2. Fundamental Methods for Characterizing Peptide Self-Assembly

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Methods to Characterize the Nanostructure and Molecular Organization of Amphiphilic Peptide Assemblies

      • V. Castelletto, I. W. Hamley
      Pages 3-21

    3. Solid-State NMR Structural Characterization of Self-Assembled Peptides with Selective 13C and 15N Isotopic Labels

      • Danting Huang, Benjamin C. Hudson, Yuan Gao, Evan K. Roberts, Anant K. Paravastu
      Pages 23-68

    4. ATR-FTIR Analysis of Amyloid Proteins

      • Jean-Marie Ruysschaert, Vincent Raussens
      Pages 69-81

    5. Imaging Protein Fibers at the Nanoscale and In Situ

      • Angelo Bella, Michael Shaw, Emiliana De Santis, Maxim G. Ryadnov
      Pages 83-100

    6. Replica Exchange Molecular Dynamics: A Practical Application Protocol with Solutions to Common Problems and a Peptide Aggregation and Self-Assembly Example

      • Ruxi Qi, Guanghong Wei, Buyong Ma, Ruth Nussinov
      Pages 101-119

    7. An Aggregate Weight-Normalized Thioflavin-T Measurement Scale for Characterizing Polymorphic Amyloids and Assembly Intermediates

      • Ronald Wetzel, Saketh Chemuru, Pinaki Misra, Ravi Kodali, Smita Mukherjee, Karunakar Kar
      Pages 121-144

    8. Nanoparticle Tracking for Protein Aggregation Research

      • Xiaomeng Lu, Regina M. Murphy
      Pages 145-158

    9. Peptide Self-Assembly Measured Using Fluorescence Correlation Spectroscopy

      • Judith J. Mittag, Joachim O. Rädler, Jennifer J. McManus
      Pages 159-171

  3. Methods for the Preparation of Functional Self-Assembled Peptide Biomaterials

    1. Front Matter

      Pages 173-173
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    2. A General Method to Prepare Peptide-Based Supramolecular Hydrogels

      • Dan Yuan, Junfeng Shi, Ning Zhou, Bing Xu
      Pages 175-180

    3. Recursive Directional Ligation Approach for Cloning Recombinant Spider Silks

      • Nina Dinjaski, Wenwen Huang, David L. Kaplan
      Pages 181-192

    4. Synthesis of Mikto-Arm Star Peptide Conjugates

      • Jin Mo Koo, Hao Su, Yi-An Lin, Honggang Cui
      Pages 193-207

    5. Synthesis and Evaluation of Self-Assembled Nanostructures of Peptide-Ï€ Chromophore Conjugates

      • Tejaswini S. Kale, John D. Tovar
      Pages 209-220

    6. Programmable Fabrication of Multilayer Collagen Nanosheets of Defined Composition

      • Tao Jiang, Vincent P. Conticello
      Pages 221-232

    7. Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies

      • Carolina Mora Solano, Yi Wen, Huifang Han, Joel H. Collier
      Pages 233-248

    8. Microwave-Assisted Synthesis and Immunological Evaluation of Self-Assembling Peptide Vaccines

      • C. Brent Chesson, Rojelio Elias Alvarado, Jai S. Rudra
      Pages 249-259

    9. Preparation and Screening of Catalytic Amyloid Assemblies

      • Zsofia Lengyel, Caroline M. Rufo, Ivan V. Korendovych
      Pages 261-270

    10. Self-assembly of Filamentous Cell Penetrating Peptides for Gene Delivery

      • Dawei Xu, Louis DeRidder, Brendan Elmore, He Dong
      Pages 271-281
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Keywords

About this book

This volume details methods and protocols on b-sheet assemblies and collagen. Divided into three parts chapters focus on expanding use of solid-state NMR as a powerful method to enhance structural understanding of self-assembled peptide materials, methods for the design, synthesis, and application of self-assembled peptide materials, and structural and mechanistic analyses of pathological amyloid systems that provide novel ways to assess function of the various possible aggregates as well to determine how the structure of these materials correlates to function/dysfunction in the biological context. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Peptide Self-Assembly: Methods and Protocols aims to capture modern methods that span the breadth of the exciting and expanding field of peptide self-assembly.


Editors and Affiliations

  • Department of Chemistry, University of Rochester, Rochester, USA

    Bradley L. Nilsson

  • Department of Medicinal Chemistry, Institute for Translational Neuroscience, University of Minnesota, Minneapolis, USA

    Todd M. Doran

Bibliographic Information

  • Book Title: Peptide Self-Assembly

  • Book Subtitle: Methods and Protocols

  • Editors: Bradley L. Nilsson, Todd M. Doran

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7811-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7809-0Published: 10 May 2018

  • Softcover ISBN: 978-1-4939-9292-8Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7811-3Published: 09 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 452

  • Number of Illustrations: 77 b/w illustrations, 53 illustrations in colour

  • Topics: Protein Science

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