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Adipose-Derived Stem Cells

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Bruce A. Bunnell

    1. Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, USA

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  2. Jeffrey M. Gimble

    1. Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, USA
      LaCell LLC, New Orleans, USA

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1773)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xii
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  2. Isolation and Flow Cytometric Analysis of the Stromal Vascular Fraction Isolated from Mouse Adipose Tissue

    • Annie C. Bowles, Alan Tucker, Bruce A. Bunnell
    Pages 1-9

  3. A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant Time Frame

    • Joel A. Aronowitz, Ryan A. Lockhart, Cloe S. Hakakian
    Pages 11-19

  4. Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem Cells

    • Christina L. Rettinger, Kai P. Leung, Rodney K. Chan
    Pages 21-30

  5. Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells

    • Anne-Laure Hafner, Tala Mohsen-Kanson, Christian Dani
    Pages 31-39

  6. Methylcellulose Based Thermally Reversible Hydrogels

    • Anoosha Forghani, Ram Devireddy
    Pages 41-51

  7. Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery

    • Pascal Morissette Martin, Arthi Shridhar, Claire Yu, Cody Brown, Lauren E. Flynn
    Pages 53-71

  8. Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells

    • Anthony D. Foster, Nicholas Clark, Thomas A. Davis
    Pages 73-91

  9. High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered Substitutes

    • Dominique Mayrand, Julie Fradette
    Pages 93-105

  10. Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery

    • Pietro Gentile, Valerio Cervelli
    Pages 107-122

  11. Myogenic Differentiation of ASCs Using Biochemical and Biophysical Induction

    • Pinar Yilgor Huri, Justin Morrissette-McAlmon, Warren L. Grayson
    Pages 123-135

  12. Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis

    • Gail Kilroy, Marilyn Dietrich, Xiying Wu, Jeffrey M. Gimble, Z. Elizabeth Floyd
    Pages 137-146

  13. Three-Dimensional Magnetic Levitation Culture System Simulating White Adipose Tissue

    • Hubert Tseng, Alexes C. Daquinag, Glauco R. Souza, Mikhail G. Kolonin
    Pages 147-154

  14. Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

    • Jie Li, J. Lowry Curley, Z. Elizabeth Floyd, Xiying Wu, Yuan Di C. Halvorsen, Jeffrey M. Gimble
    Pages 155-165

  15. Isolation of Murine Adipose-Derived Stromal/Stem Cells Using an Explant Culture Method

    • Jie Li, Hui Li, Weidong Tian
    Pages 167-171

  16. Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation

    • Carmel Fargason, Nan Zhang, Mandi J. Lopez
    Pages 173-188

  17. Canine Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Characterization

    • Wei Duan, Mandi J. Lopez
    Pages 189-202

  18. Soft Tissue Reconstruction

    • Francesco Egro, Kacey G. Marra
    Pages 203-213

  19. Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain

    • Rachel C. Nordberg, Josie C. Bodle, Elizabeth G. Loboa
    Pages 215-230

  20. Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells

    • Shahensha Shaik, Ram Devireddy
    Pages 231-259
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Keywords

About this book

This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls.



Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more aboutthe scientific advances in the field of ASC biology and application.


Editors and Affiliations

  • Center for Stem Cell Research and Regenerative Medicine, Tulane University School of Medicine, New Orleans, USA

    Bruce A. Bunnell, Jeffrey M. Gimble

  • LaCell LLC, New Orleans, USA

    Jeffrey M. Gimble

Bibliographic Information

  • Book Title: Adipose-Derived Stem Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Bruce A. Bunnell, Jeffrey M. Gimble

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7799-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7797-0Published: 24 April 2018

  • Softcover ISBN: 978-1-4939-9288-1Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7799-4Published: 23 April 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 284

  • Number of Illustrations: 10 b/w illustrations, 46 illustrations in colour

  • Topics: Cell Biology

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