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Cell-Based Microarrays

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Peter Ertl

    1. Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Mario Rothbauer

    1. Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1771)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Next-Generation Live-Cell Microarray Technologies

      • Mario Rothbauer, Peter Ertl
      Pages 3-8

  3. Biointerface Engineering and Live-Cell Microarrays

    1. Front Matter

      Pages 9-9
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    2. Cell-Based Microarrays Using Superhydrophobic Platforms Patterned with Wettable Regions

      • Mariana B. Oliveira, João F. Mano
      Pages 11-26

    3. 3D Plasma Nanotextured® Polymeric Surfaces for Protein or Antibody Arrays, and Biomolecule and Cell Patterning

      • Katerina Tsougeni, Kosmas Ellinas, George Koukouvinos, Panagiota S. Petrou, Angeliki Tserepi, Sotirios E. Kakabakos et al.
      Pages 27-40

    4. Single Cell Microarrays Fabricated by Microscale Plasma-Initiated Protein Patterning (μPIPP)

      • Anita Reiser, Matthias Lawrence Zorn, Alexandra Murschhauser, Joachim Oskar Rädler
      Pages 41-54

    5. Artificial Microniche Array with Spatially Structured Biochemical Cues

      • Xumei Gao, Celine Stoecklin, Yue Zhang, Zhe Weng, Richard De Mets, Gianluca Grenci et al.
      Pages 55-66

  4. Printing Techniques for Live-Cell Microarrays

    1. Front Matter

      Pages 67-67
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    2. Three-Dimensional Cell-Based Microarrays: Printing Pluripotent Stem Cells into 3D Microenvironments

      • Jorge F. Pascoal, Tiago G. Fernandes, Gregory J. Nierode, Maria Margarida Diogo, Jonathan S. Dordick, Joaquim M. S. Cabral
      Pages 69-81

    3. Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printing

      • Julie Foncy, Aurore Estève, Amélie Degache, Camille Colin, Jean Christophe Cau, Laurent Malaquin et al.
      Pages 83-95

    4. Hydrogel Printing Based on UV-Induced Projection for Cell-Based Microarray Fabrication

      • Wenguang Yang, Haibo Yu, Yuechao Wang, Lianqing Liu
      Pages 97-105

    5. Inkjet Printing for Biomedical Applications

      • Christopher Chi Wai Tse, Patrick J. Smith
      Pages 107-117

  5. Live-Cell Microarray Application

    1. Front Matter

      Pages 119-119
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    2. High-Throughput Screening of Senescence Markers in Hematopoietic Stem Cells Derived from Induced Pluripotent Stem Cells

      • Shyam Sushama Jose, Kamila Bendickova, Jan Fric
      Pages 121-130

    3. Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems

      • Rebecca Jonczyk, Thomas Scheper, Frank Stahl
      Pages 131-145

    4. Fabrication of Multielectrode Arrays for Neurobiology Applications

      • Mario Malerba, Hayder Amin, Gian N. Angotzi, Alessandro Maccione, Luca Berdondini
      Pages 147-157

  6. Microfluidic Live-Cell Arrays

    1. Front Matter

      Pages 159-159
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    2. Rapid Prototyping of Thermoplastic Microfluidic Devices

      • Richard Novak, Carlos F. Ng, Donald E. Ingber
      Pages 161-170

    3. Fabrication of Biomolecule Microarrays Using Rapid Photochemical Surface Patterning in Thiol–Ene-Based Microfluidic Devices

      • Alexander Jönsson, Josiane P. Lafleur
      Pages 171-182
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Keywords

About this book

This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell microarrays, printing techniques for live-cell microarrays, live-cell microarray application, microfluidic live-cell arrays, and advanced materials suitable for live-cell microarrays. Some of the topics covered are artificial micro-niche array with spatially structured biochemical cues; inkjet printing for biomedical application; fabrication of multi-electrode arrays for neurobiology applications; rapid prototyping of thermoplastic microfluidic devices; and oxygen-generating photocrosslinkable hydrogel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cell-Based Microarrays: Methods and Protocols is a valuable resource for anyone interested in expanding their knowledge and research of cell-based microarrays.

Editors and Affiliations

  • Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    Peter Ertl, Mario Rothbauer

Bibliographic Information

  • Book Title: Cell-Based Microarrays

  • Book Subtitle: Methods and Protocols

  • Editors: Peter Ertl, Mario Rothbauer

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7792-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7791-8Published: 10 April 2018

  • Softcover ISBN: 978-1-4939-9286-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7792-5Published: 09 April 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 260

  • Number of Illustrations: 17 b/w illustrations, 66 illustrations in colour

  • Topics: Cell Biology

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