Skip to main content
SpringerLink
Log in

Digital PCR

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. George Karlin-Neumann

    1. Digital Biology Center, Bio-Rad Laboratories, Pleasanton, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Francisco Bizouarn

    1. Digital Biology Center, Bio-Rad Laboratories, Pleasanton, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1768)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 189.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 249.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Background Concepts

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Entering the Pantheon of 21st Century Molecular Biology Tools: A Perspective on Digital PCR

      • George Karlin-Neumann, Francisco Bizouarn
      Pages 3-10

    3. Basic Concepts and Validation of Digital PCR Measurements

      • Leonardo Pinheiro, Kerry R. Emslie
      Pages 11-24

    4. Fundamentals of Counting Statistics in Digital PCR: I Just Measured Two Target Copies–What Does It Mean?

      • Svilen Tzonev
      Pages 25-43

    5. Control Materials and Digital PCR Methods for Evaluation of Circulating Cell-Free DNA Extractions from Plasma

      • Alexandra S. Whale, Ana Fernandez-Gonzalez, Alice Gutteridge, Alison S. Devonshire
      Pages 45-65

  3. Absolute Quantification

    1. Front Matter

      Pages 67-67
      Download chapter PDF

    2. Multiplex Droplet Digital PCR Protocols for Quantification of GM Maize Events

      • David Dobnik, Bjørn Spilsberg, Alexandra Bogožalec Košir, Dejan Štebih, Dany Morisset, Arne Holst-Jensen et al.
      Pages 69-98

    3. Using Droplet Digital PCR to Detect Coinfection of Human Herpesviruses 6A and 6B (HHV-6A and HHV-6B) in Clinical Samples

      • Ashley Vellucci, Emily C. Leibovitch, Steven Jacobson
      Pages 99-109

    4. Biomarkers in Cerebrospinal Fluid: Analysis of Cell-Free Circulating Mitochondrial DNA by Digital PCR

      • Petar Podlesniy, Ramon Trullas
      Pages 111-126

    5. Testing of General and Human-Associated Fecal Contamination in Waters

      • Yiping Cao, Meredith R. Raith, John F. Griffith
      Pages 127-140

  4. Copy Number Variation

    1. Front Matter

      Pages 141-141
      Download chapter PDF

    2. Analyzing Copy Number Variation with Droplet Digital PCR

      • Avery Davis Bell, Christina L. Usher, Steven A. McCarroll
      Pages 143-160

    3. Assessing HER2 Amplification in Plasma cfDNA

      • Isaac Garcia-Murillas, Nicholas C. Turner
      Pages 161-172

    4. Detection and Quantification of Mosaic Genomic DNA Variation in Primary Somatic Tissues Using ddPCR: Analysis of Mosaic Transposable-Element Insertions, Copy-Number Variants, and Single-Nucleotide Variants

      • Bo Zhou, Michael S. Haney, Xiaowei Zhu, Reenal Pattni, Alexej Abyzov, Alexander E. Urban
      Pages 173-190

  5. Rare Mutation and Rare Allele Detection

    1. Front Matter

      Pages 191-191
      Download chapter PDF

    2. Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer Using Droplet Digital PCR

      • Yanan Kuang, Allison O’Connell, Adrian G. Sacher, Nora Feeney, Ryan S. Alden, Geoffrey R. Oxnard et al.
      Pages 193-207

    3. Detection of Cancer DNA in Early Stage and Metastatic Breast Cancer Patients

      • Arielle J. Medford, Riaz N. Gillani, Ben Ho Park
      Pages 209-227

    4. Droplet Digital PCR for Minimal Residual Disease Detection in Mature Lymphoproliferative Disorders

      • Daniela Drandi, Simone Ferrero, Marco Ladetto
      Pages 229-256

    5. Quantitation of JAK2 V617F Allele Burden by Using the QuantStudio™ 3D Digital PCR System

      • Elena Kinz, Axel Muendlein
      Pages 257-273
Back to top

Keywords

About this book

This volume explores and explains how digital PCRs (dPCRs) help in the study of numerous topics, such as infectious diseases, evolution of cancer and treatment responses, somatic mosaicism, genome editing and cell therapy, and food testing for GMOs and pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Authoritative and thorough, Digital PCR: Methods and Protocols is a valuable resource for specialists in various fields, including geneticists, neurologists, immunologists, oncologists, and researchers who are interested in environmental sciences.”


Reviews

“The editors of the volume have succeeded in providing specialists in different sectors with a wide range of technology applications that can be adapted to other platforms or can provide the basis for creating new ones.” (Paolo Catarsi, European Journal of Histochemistry, Vol. 63, 2019)

Editors and Affiliations

  • Digital Biology Center, Bio-Rad Laboratories, Pleasanton, USA

    George Karlin-Neumann, Francisco Bizouarn

Bibliographic Information

  • Book Title: Digital PCR

  • Book Subtitle: Methods and Protocols

  • Editors: George Karlin-Neumann, Francisco Bizouarn

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7778-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7776-5Published: 01 May 2018

  • Softcover ISBN: 978-1-4939-9282-9Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7778-9Published: 01 May 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 557

  • Number of Illustrations: 13 b/w illustrations, 128 illustrations in colour

  • Topics: Human Genetics

Publish with us

Policies and ethics

Back to top

Search

Navigation