Skip to main content
SpringerLink
Log in
Book cover

Epigenome Editing

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Albert Jeltsch

    1. Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, Stuttgart, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Marianne G. Rots

    1. Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques for the study of epigenome editing
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1767)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. General Reviews

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Editing the Epigenome: Overview, Open Questions, and Directions of Future Development

      • Marianne G. Rots, Albert Jeltsch
      Pages 3-18

    3. Zinc Fingers, TALEs, and CRISPR Systems: A Comparison of Tools for Epigenome Editing

      • Charlene Babra Waryah, Colette Moses, Mahira Arooj, Pilar Blancafort
      Pages 19-63

    4. Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities

      • Dilara Sen, Albert J. Keung
      Pages 65-87

    5. Generation of TALE-Based Designer Epigenome Modifiers

      • Sandra Nitsch, Claudio Mussolino
      Pages 89-109

  3. Topical Reviews

    1. Front Matter

      Pages 111-111
      Download chapter PDF

    2. Neuroepigenetic Editing

      • Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, Elizabeth A. Heller
      Pages 113-136

    3. Allele-Specific Epigenome Editing

      • Pavel Bashtrykov, Albert Jeltsch
      Pages 137-146

    4. Key to Delivery: The (Epi-)genome Editing Vector Toolbox

      • Sabrina Just, Hildegard Büning
      Pages 147-166

    5. CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control

      • Rutger A. F. Gjaltema, Edda G. Schulz
      Pages 167-185

  4. Delivery Protocols

    1. Front Matter

      Pages 187-187
      Download chapter PDF

    2. Delivery of Designer Epigenome Modifiers into Primary Human T Cells

      • Tafadzwa Mlambo, Marianna Romito, Tatjana I. Cornu, Claudio Mussolino
      Pages 189-203

    3. Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques

      • Peter J. Hamilton, Carissa J. Lim, Eric J. Nestler, Elizabeth A. Heller
      Pages 205-214

    4. Stable Expression of Epigenome Editors via Viral Delivery and Genomic Integration

      • Carolin Kroll, Philipp Rathert
      Pages 215-225

    5. Purified Protein Delivery to Activate an Epigenetically Silenced Allele in Mouse Brain

      • Benjamin Pyles, Barbara J. Bailus, Henriette O’Geen, David J. Segal
      Pages 227-239

    6. Non-viral Methodology for Efficient Co-transfection

      • Jessica A. Kretzmann, Cameron W. Evans, Marck Norret, Pilar Blancafort, K. Swaminathan Iyer
      Pages 241-254

  5. Histone Editing Readout Protocols

    1. Front Matter

      Pages 255-255
      Download chapter PDF

    2. Chromatin Immunoprecipitation in Human and Yeast Cells

      • Jessica B. Lee, Albert J. Keung
      Pages 257-269

    3. Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis

      • Darren K. Patten, Giacomo Corleone, Luca Magnani
      Pages 271-288
Back to top

Keywords

About this book

This detailed book explores the concepts and applications of epigenome editing, as presented by leading scientists in the field. Beginning with some general and topical reviews, the collection continues by covering the design of DNA-binding devices, optimization of the effector domains, readout of epigenome marks, and approaches for delivery at the cellular and organismal level. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Epigenome Editing: Methods and Protocols will be of great assistance to people new to the field but also to those already engaged, as epigenetic editing is still a relatively unexplored field with many issues to be resolved.

Editors and Affiliations

  • Department of Biochemistry, Institute of Biochemistry and Technical Biochemistry, Stuttgart, Germany

    Albert Jeltsch

  • Department of Pathology and Medical Biology, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands

    Marianne G. Rots

Bibliographic Information

  • Book Title: Epigenome Editing

  • Book Subtitle: Methods and Protocols

  • Editors: Albert Jeltsch, Marianne G. Rots

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7774-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7773-4Published: 10 March 2018

  • Softcover ISBN: 978-1-4939-9281-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7774-1Published: 09 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 482

  • Number of Illustrations: 14 b/w illustrations, 69 illustrations in colour

  • Topics: Human Genetics

Publish with us

Policies and ethics

Back to top

Search

Navigation