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Intravital Imaging of Dynamic Bone and Immune Systems

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Masaru Ishii

    1. Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka, Japan

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  • Includes cutting-edge methods and protocols for the use of intravital imaging
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1763)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Bone Imaging: Osteoclast and Osteoblast Dynamics

    • Junichi Kikuta, Masaru Ishii
    Pages 1-9

  3. Intravital Imaging of Mouse Bone Marrow: Hemodynamics and Vascular Permeability

    • Yookyung Jung, Joel A. Spencer, Anthony P. Raphael, Juwell W. Wu, Clemens Alt, Judith R. Runnels et al.
    Pages 11-22

  4. Bone Imaging: Platelet Formation Dynamics

    • Asuka Sakata, Satoshi Nishimura
    Pages 23-28

  5. Live Imaging of Interstitial T Cell Migration Using Lymph Node Slices

    • Tomoya Katakai
    Pages 29-42

  6. Two-Photon Imaging of T-Cell Motility in Lymph Nodes: In Vivo and Ex Vivo Approaches

    • Akira Takeda, Masayuki Miyasaka, Eiji Umemoto
    Pages 43-52

  7. Imaging the Lymph Node Stroma

    • Clément Ghigo, Rebecca Gentek, Marc Bajénoff
    Pages 53-61

  8. Intravital Imaging of B Cell Responses in Lymph Nodes

    • Stefano Sammicheli, Mirela Kuka, Matteo Iannacone
    Pages 63-74

  9. Live Imaging of the Skin Immune Responses: Visualization of the Contact Hypersensitivity Response

    • Gyohei Egawa, Tetsuya Honda, Kenji Kabashima
    Pages 75-85

  10. Imaging of Inflammatory Responses in the Mouse Ear Skin

    • Jackson LiangYao Li, Chi Ching Goh, Lai Guan Ng
    Pages 87-107

  11. In Vivo Imaging of Immune Cells in Peyer’s Patches

    • Andrea Reboldi
    Pages 109-118

  12. Intravital Imaging of T Cells Within the Spinal Cord

    • Naoto Kawakami
    Pages 119-127

  13. Kidney Imaging: Intravital Microscopy

    • Takashi Hato, Seth Winfree, Pierre C. Dagher
    Pages 129-136

  14. Intravital Imaging of Liver Cell Dynamics

    • Sayaka Matsumoto, Junichi Kikuta, Masaru Ishii
    Pages 137-143

  15. Intravital Imaging of the Heart at the Cellular Level Using Two-Photon Microscopy

    • Ryohei Matsuura, Shigeru Miyagawa, Junichi Kikuta, Masaru Ishii, Yoshiki Sawa
    Pages 145-151

  16. Imaging Window Device for Subcutaneous Implantation Tumor

    • Wataru Ikeda, Ken Sasai, Tsuyoshi Akagi
    Pages 153-163

  17. New Tools for Imaging of Immune Systems: Visualization of Cell Cycle, Cell Death, and Cell Movement by Using the Mice Lines Expressing Fucci, SCAT3.1, and Kaede and KikGR

    • Michio Tomura
    Pages 165-174

  18. Back Matter

    Pages 175-175
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Keywords

About this book

This detailed volume presents a number of chapters that will aid researchers in jumping the hurdle preventing the popularization of advanced intravital imaging technology usage, namely the complicated experimental protocols. Leading researchers undertaking imaging studies in the field of bone and immune systems have contributed the chapters that aim to guide readers through these complex methodologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Intravital Imaging of Dynamic Bone and Immune Systems: Methods and Protocols will serve as an idea guide for general readers in order to establish and perform these experiments by their own. 

Editors and Affiliations

  • Department of Immunology and Cell Biology, Graduate School of Medicine, Osaka University, Osaka, Japan

    Masaru Ishii

Bibliographic Information

  • Book Title: Intravital Imaging of Dynamic Bone and Immune Systems

  • Book Subtitle: Methods and Protocols

  • Editors: Masaru Ishii

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7762-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7761-1Published: 24 February 2018

  • Softcover ISBN: 978-1-4939-8544-9Published: 06 June 2019

  • eBook ISBN: 978-1-4939-7762-8Published: 23 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 175

  • Number of Illustrations: 10 b/w illustrations, 56 illustrations in colour

  • Topics: Imaging / Radiology

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