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Protein Complex Assembly

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Joseph A. Marsh

    1. MRC Human Genetics Unit, Institute of Genetics & Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1764)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Experimental Methods

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Experimental Characterization of Protein Complex Structure, Dynamics, and Assembly

      • Jonathan N. Wells, Joseph A. Marsh
      Pages 3-27

    3. High-Throughput Electron Cryo-tomography of Protein Complexes and Their Assembly

      • Louie D. Henderson, Morgan Beeby
      Pages 29-44

    4. Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy

      • Brian L. Gilmore, A. Cameron Varano, William Dearnaley, Yanping Liang, Bridget C. Marcinkowski, Madeline J. Dukes et al.
      Pages 45-58

    5. Time-Resolved Cryo-electron Microscopy Using a Microfluidic Chip

      • Sandip Kaledhonkar, Ziao Fu, Howard White, Joachim Frank
      Pages 59-71

    6. Characterizing Protein-Protein Interactions Using Solution NMR Spectroscopy

      • Jose Luis Ortega-Roldan, Martin Blackledge, Malene Ringkjøbing Jensen
      Pages 73-85

    7. Reconstitution of Isotopically Labeled Ribosomal Protein L29 in the 50S Large Ribosomal Subunit for Solution-State and Solid-State NMR

      • Emeline Barbet-Massin, Eli van der Sluis, Joanna Musial, Roland Beckmann, Bernd Reif
      Pages 87-100

    8. Characterizing Protein-Protein Interactions Using Deep Sequencing Coupled to Yeast Surface Display

      • Angelica V. Medina-Cucurella, Timothy A. Whitehead
      Pages 101-121

    9. Structurally Guided In Vivo Crosslinking

      • Johanna C. Scheinost, Thomas G. Gligoris
      Pages 123-132

    10. Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry

      • Elisabetta Boeri Erba, Luca Signor, Mizar F. Oliva, Fabienne Hans, Carlo Petosa
      Pages 133-151

    11. Hydrogen-Deuterium Exchange Mass Spectrometry to Study Protein Complexes

      • Brent A. Kochert, Roxana E. Iacob, Thomas E. Wales, Alexandros Makriyannis, John R. Engen
      Pages 153-171

    12. Structural Analysis of Protein Complexes by Cross-Linking and Mass Spectrometry

      • Moriya Slavin, Nir Kalisman
      Pages 173-183

    13. Global Characterization of Protein Complexes by Biochemical Purification-Mass Spectrometry (BP/MS)

      • Reza Pourhaghighi, Andrew Emili
      Pages 185-191

    14. Proteomic Profiling of Integrin Adhesion Complex Assembly

      • Adam Byron
      Pages 193-236

    15. Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies

      • Philipp Hoess, Markus Mund, Manuel Reitberger, Jonas Ries
      Pages 237-251

    16. Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion

      • Shannon J L. Pinnington, John F. Marshall, Ann P. Wheeler
      Pages 253-265

    17. Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI

      • Fabian Hertel, Gary C. H. Mo, Peter Dedecker, Jin Zhang
      Pages 267-277

    18. Detecting Purinosome Metabolon Formation with Fluorescence Microscopy

      • Anthony M. Pedley, Stephen J. Benkovic
      Pages 279-289

    19. Analysis of Bacterial Pilus Assembly by Shearing and Immunofluorescence Microscopy

      • Areli Luna-Rico, Jenny-Lee Thomassin, Olivera Francetic
      Pages 291-305
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Keywords

About this book

This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • MRC Human Genetics Unit, Institute of Genetics & Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom

    Joseph A. Marsh

Bibliographic Information

  • Book Title: Protein Complex Assembly

  • Book Subtitle: Methods and Protocols

  • Editors: Joseph A. Marsh

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7759-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7758-1Published: 01 April 2018

  • Softcover ISBN: 978-1-4939-9277-5Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7759-8Published: 31 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 506

  • Number of Illustrations: 14 b/w illustrations, 79 illustrations in colour

  • Topics: Protein Science

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