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  • © 2018

Mitophagy

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1759)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. Short Overview

    • Norihiko Furuya
    Pages 3-8
  3. Induction of PINK1/Parkin-Mediated Mitophagy

    • Shigeto Sato, Norihiko Furuya
    Pages 9-17
  4. Immunocytochemical Monitoring of PINK1/Parkin-Mediated Mitophagy in Cultured Cells

    • Motoki Fujimaki, Shinji Saiki, Yukiko Sasazawa, Kei-Ichi Ishikawa, Yoko Imamichi, Katsuhiko Sumiyoshi et al.
    Pages 19-27
  5. Observation of Parkin-Mediated Mitophagy in Pancreatic β-Cells

    • Atsushi Hoshino, Satoaki Matoba
    Pages 41-46
  6. Monitoring Mitochondrial Changes by Alteration of the PINK1-Parkin Signaling in Drosophila

    • Tsuyoshi Inoshita, Kahori Shiba-Fukushima, Hongrui Meng, Nobutaka Hattori, Yuzuru Imai
    Pages 47-57
  7. Assessment of Mitophagy in iPS Cell-Derived Neurons

    • Kei-Ichi Ishikawa, Akihiro Yamaguchi, Hideyuki Okano, Wado Akamatsu
    Pages 59-67
  8. MitoPho8Δ60 Assay as a Tool to Quantitatively Measure Mitophagy Activity

    • Zhiyuan Yao, Xu Liu, Daniel J. Klionsky
    Pages 85-93
  9. Mitophagy in Yeast: A Screen of Mitophagy-Deficient Mutants

    • Kentaro Furukawa, Tomotake Kanki
    Pages 95-104
  10. Flow Cytometer Monitoring of Bnip3- and Bnip3L/Nix-Dependent Mitophagy

    • Matilda Å prung, Ivan Dikic, Ivana Novak
    Pages 105-110
  11. Exploring MicroRNAs on NIX-Dependent Mitophagy

    • Wen Li, Hao Chen, Shupeng Li, Guanghong Lin, Du Feng
    Pages 111-121
  12. Monitoring of Atg5-Independent Mitophagy

    • Satoko Arakawa, Shinya Honda, Satoru Torii, Masatsune Tsujioka, Shigeomi Shimizu
    Pages 125-132
  13. Monitoring Mitophagy During Aging in Caenorhabditis elegans

    • Nikolaos Charmpilas, Konstantinos Kounakis, Nektarios Tavernarakis
    Pages 151-160
  14. Dopaminergic Neuron-Specific Autophagy-Deficient Mice

    • Shigeto Sato, Nobutaka Hattori
    Pages 173-175
  15. Back Matter

    Pages 177-180

About this book

This volume focuses on mitophagy providing methods and protocols on the methodologies and the background information on monitoring mitophagy. Chapters detail the analysis of mitophagy in connection with Parkinson’s disease, especially young onset Parkinson’s disease impaired by PINK/parkin pathway, Drosophila, yeast, and Caenorhabditis elegans models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Mitophagy: Methods and Protocols aims to stimulate and encourage researchers to carry out ongoing and new investigations into mitophagy.

Editors and Affiliations

  • Department of Neurology, Juntendo University School of Medicine, Tokyo, Japan

    Nobutaka Hattori, Shinji Saiki

Bibliographic Information

  • Book Title: Mitophagy

  • Book Subtitle: Methods and Protocols

  • Editors: Nobutaka Hattori, Shinji Saiki

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7750-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7749-9Published: 21 August 2018

  • Softcover ISBN: 978-1-4939-9274-4Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7750-5Published: 20 August 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 180

  • Topics: Cell Biology

Buy it now

Buying options

eBook USD 59.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 79.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access