Reporter Gene Assays

1. Front Matter

   Pages i-xii

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2. Genome-Edited Cell Lines for High-Throughput Screening

   • Patricia Dranchak, John J. Moran, Ryan MacArthur, Camila Lopez-Anido, James Inglese, John Svaren

   Pages 1-17

3. High-Throughput Firefly Luciferase Reporter Assays

   • Ellen Siebring-van Olst, Victor W. van Beusechem

   Pages 19-29

4. Using the 2A Protein Coexpression System: Multicistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins

   • Garry A. Luke, Martin D. Ryan

   Pages 31-48

5. Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter

   • Chidambaram Ramanathan, Andrew C. Liu

   Pages 49-64

6. High-Throughput Screening Method to Identify Alternative Splicing Regulators

   • Cheryl Stork, Sika Zheng

   Pages 65-74

7. High-Throughput Screening of a Luciferase Reporter of Gene Silencing on the Inactive X Chromosome

   • Alissa Keegan, Kathrin Plath, Robert Damoiseaux

   Pages 75-87

8. Making It All Work: Functional Genomics and Reporter Gene Assays

   • Genevieve Welch, Robert Damoiseaux, Loren Miraglia

   Pages 89-105

9. Reporter Gene Assays Using Transfectable Functional Genomics Libraries

   • Genevieve Welch, Robert Damoiseaux, Loren Miraglia

   Pages 107-120

10. Reporter Gene Assays Using Viral Functional Genomics Libraries

    • Genevieve Welch, Robert Damoiseaux, Loren Miraglia

    Pages 121-133

11. Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii

    • Crysten E. Blaby-Haas, M. Dudley Page, Sabeeha S. Merchant

    Pages 135-148

12. Gene Expression Analysis by Arylsulfatase Assays in the Green Alga Chlamydomonas reinhardtii

    • Ian K. Blaby, Crysten E. Blaby-Haas

    Pages 149-161

13. Endogenous Locus Reporter Assays

    • Yaping Liu, Jeffrey Hermes, Jing Li, Matthew Tudor

    Pages 163-177

14. High-Content Reporter Assays

    • Erica Cook, Jeffrey Hermes, Jing Li, Matthew Tudor

    Pages 179-195

15. Data Mining and Computational Modeling of High-Throughput Screening Datasets

    • Sean Ekins, Alex M. Clark, Krishna Dole, Kellan Gregory, Andrew M. Mcnutt, Anna Coulon Spektor et al.

    Pages 197-221

16. Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscopy

    • Nathan Y. Bentolila, Raymond L. Barnhill, Claire Lugassy, Laurent A. Bentolila

    Pages 223-232

17. Back Matter

    Pages 233-234

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This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs.  Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interestedin learning more about reporter genes.

• genes
• gene expression patterns
• cellular functions
• NFkB activation in immune cells
• high-throughput drug screening
• pathogenic protein expression

• Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, USA

  Robert Damoiseaux

• Pfizer, Inc., Cambridge, USA

  Samuel Hasson

Policies and ethics