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Reporter Gene Assays

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Robert Damoiseaux

    1. Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Samuel Hasson

    1. Pfizer, Inc., Cambridge, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1755)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Genome-Edited Cell Lines for High-Throughput Screening

    • Patricia Dranchak, John J. Moran, Ryan MacArthur, Camila Lopez-Anido, James Inglese, John Svaren
    Pages 1-17

  3. High-Throughput Firefly Luciferase Reporter Assays

    • Ellen Siebring-van Olst, Victor W. van Beusechem
    Pages 19-29

  4. Using the 2A Protein Coexpression System: Multicistronic 2A Vectors Expressing Gene(s) of Interest and Reporter Proteins

    • Garry A. Luke, Martin D. Ryan
    Pages 31-48

  5. Developing Mammalian Cellular Clock Models Using Firefly Luciferase Reporter

    • Chidambaram Ramanathan, Andrew C. Liu
    Pages 49-64

  6. High-Throughput Screening Method to Identify Alternative Splicing Regulators

    • Cheryl Stork, Sika Zheng
    Pages 65-74

  7. High-Throughput Screening of a Luciferase Reporter of Gene Silencing on the Inactive X Chromosome

    • Alissa Keegan, Kathrin Plath, Robert Damoiseaux
    Pages 75-87

  8. Making It All Work: Functional Genomics and Reporter Gene Assays

    • Genevieve Welch, Robert Damoiseaux, Loren Miraglia
    Pages 89-105

  9. Reporter Gene Assays Using Transfectable Functional Genomics Libraries

    • Genevieve Welch, Robert Damoiseaux, Loren Miraglia
    Pages 107-120

  10. Reporter Gene Assays Using Viral Functional Genomics Libraries

    • Genevieve Welch, Robert Damoiseaux, Loren Miraglia
    Pages 121-133

  11. Using YFP as a Reporter of Gene Expression in the Green Alga Chlamydomonas reinhardtii

    • Crysten E. Blaby-Haas, M. Dudley Page, Sabeeha S. Merchant
    Pages 135-148

  12. Gene Expression Analysis by Arylsulfatase Assays in the Green Alga Chlamydomonas reinhardtii

    • Ian K. Blaby, Crysten E. Blaby-Haas
    Pages 149-161

  13. Endogenous Locus Reporter Assays

    • Yaping Liu, Jeffrey Hermes, Jing Li, Matthew Tudor
    Pages 163-177

  14. High-Content Reporter Assays

    • Erica Cook, Jeffrey Hermes, Jing Li, Matthew Tudor
    Pages 179-195

  15. Data Mining and Computational Modeling of High-Throughput Screening Datasets

    • Sean Ekins, Alex M. Clark, Krishna Dole, Kellan Gregory, Andrew M. Mcnutt, Anna Coulon Spektor et al.
    Pages 197-221

  16. Intravital Imaging of Human Melanoma Cells in the Mouse Ear Skin by Two-Photon Excitation Microscopy

    • Nathan Y. Bentolila, Raymond L. Barnhill, Claire Lugassy, Laurent A. Bentolila
    Pages 223-232

  17. Back Matter

    Pages 233-234
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Keywords

About this book

This volume explores the applications of reporter gene technology and the methodologies needed for their effective implementation. The chapters in this book cover practical topics such as how to integrate reporter constructs into cellular models, viral delivery, splicing applications, in vivo imaging, and a guide to the use of multi-cistronic constructs.  Additionally, chapters also include detailed mechanistic uses of reporter genes in cellular pathways, and a look at project and data management of screening applications.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and thorough, Reporter Gene Assays: Methods and Protocols is a valuable resource for anyone who is interestedin learning more about reporter genes.


Editors and Affiliations

  • Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, USA

    Robert Damoiseaux

  • Pfizer, Inc., Cambridge, USA

    Samuel Hasson

Bibliographic Information

  • Book Title: Reporter Gene Assays

  • Book Subtitle: Methods and Protocols

  • Editors: Robert Damoiseaux, Samuel Hasson

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7724-6

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7722-2Published: 19 April 2018

  • Softcover ISBN: 978-1-4939-9268-3Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7724-6Published: 18 April 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 234

  • Number of Illustrations: 11 b/w illustrations, 42 illustrations in colour

  • Topics: Human Genetics

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