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Mouse Embryogenesis

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Paul Delgado-Olguin

    1. Translational Medicine, The Hospital for Sick Children, Toronto, Canada

    View editor publications

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1752)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Mouse Genotyping

    • Sandra Vuong, Paul Delgado-Olguin
    Pages 1-9

  3. Visualizing the Vascular Network in the Mouse Embryo and Yolk Sac

    • Anna R. Roy, Paul Delgado-Olguin
    Pages 11-16

  4. In Vivo Evaluation of the Cardiovascular System of Mouse Embryo and Fetus Using High Frequency Ultrasound

    • Yu-Qing Zhou, Lindsay S. Cahill, John G. Sled
    Pages 17-39

  5. Dynamic Imaging of Mouse Embryos and Cardiodynamics in Static Culture

    • Andrew L. Lopez III, Irina V. Larina
    Pages 41-52

  6. In Vivo Imaging of the Mouse Reproductive Organs, Embryo Transfer, and Oviduct Cilia Dynamics Using Optical Coherence Tomography

    • Shang Wang, Irina V. Larina
    Pages 53-62

  7. Live Imaging of Fetal Intra-abdominal Organs Using Two-Photon Laser-Scanning Microscopy

    • Yuhki Koike, Bo Li, Yong Chen, Hiromu Miyake, Carol Lee, Lijun Chi et al.
    Pages 63-69

  8. Embryonary Mouse Cardiac Fibroblast Isolation

    • Alejandra Garate-Carrillo, Israel Ramirez
    Pages 71-79

  9. Explant Culture for Studying Lung Development

    • Behzad Yeganeh, Claudia Bilodeau, Martin Post
    Pages 81-90

  10. Isolating Embryonic Cardiac Progenitors and Cardiac Myocytes by Fluorescence-Activated Cell Sorting

    • Abdalla Ahmed, Paul Delgado-Olguin
    Pages 91-100

  11. Isolation and Culture of Mouse Placental Endothelial Cells

    • Lijun Chi, Paul Delgado-Olguin
    Pages 101-109

  12. Flow Cytometry and Lineage Tracing Study for Identification of Adipocyte Precursor Cell (APC) Populations

    • Ju Hee Lee, Azadeh Yeganeh, Hisato Konoeda, Joon Ho Moon, Hoon-Ki Sung
    Pages 111-121

  13. Whole-Mount and Section In Situ Hybridization in Mouse Embryos for Detecting mRNA Expression and Localization

    • Kazuko Koshiba-Takeuchi
    Pages 123-131

  14. Chromosome Painting of Mouse Chromosomes

    • Lisa L. Hua, Takashi Mikawa
    Pages 133-143

  15. Chromatin Immunoprecipitation in Early Mouse Embryos

    • Estela G. García-González, Bladimir Roque-Ramirez, Carlos Palma-Flores, J. Manuel Hernández-Hernández
    Pages 145-155

  16. Shaping Up the Embryo: The Role of Genome 3D Organization

    • Karina Jácome-López, Mayra Furlan-Magaril
    Pages 157-175

  17. Genome Editing During Development Using the CRISPR-Cas Technology

    • Rodrigo G. Arzate-Mejía, Paula Licona-Limón, Félix Recillas-Targa
    Pages 177-190

  18. Back Matter

    Pages 191-193
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Keywords

About this book

This volume explores protocols for identifying mutant mice and characterizing parts of their anatomical, functional, cellular, and molecular phenotypes. The chapters in this book look at anatomical and functional phenotyping using quantitative imaging, isolation of specific embryonic cell types for cell culture, analysis of gene expression, and how to define chromatin structure. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Mouse Embryogenesis: Methods and Protocols is a valuable resource for experimentalists interested in discovering new aspects of embryogenesis control, organ function, and the origin of disease.



Editors and Affiliations

  • Translational Medicine, The Hospital for Sick Children, Toronto, Canada

    Paul Delgado-Olguin

Bibliographic Information

  • Book Title: Mouse Embryogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Paul Delgado-Olguin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7714-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7713-0Published: 22 March 2018

  • Softcover ISBN: 978-1-4939-9265-2Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7714-7Published: 21 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 193

  • Number of Illustrations: 3 b/w illustrations, 46 illustrations in colour

  • Topics: Cell Biology

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