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Cell Migration

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Alexis Gautreau

    1. Laboratory of Biochemistry, Ecole Polytechnique, Palaiseau, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1749)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Random Migration Assays of Mammalian Cells and Quantitative Analyses of Single Cell Trajectories

    • Irene Dang, Alexis Gautreau
    Pages 1-9

  3. Directional Collective Migration in Wound Healing Assays

    • Nicolas Molinie, Alexis Gautreau
    Pages 11-19

  4. An In Vitro System to Study the Mesenchymal-to-Amoeboid Transition

    • Aleksandra S. Chikina, Antonina Y. Alexandrova
    Pages 21-27

  5. An In Vitro System to Study the Epithelial–Mesenchymal Transition In Vitro

    • Natalya A. Gloushankova, Svetlana N. Rubtsova, Irina Y. Zhitnyak
    Pages 29-42

  6. Detection of Migrasomes

    • Yang Chen, Ying Li, Liang Ma, Li Yu
    Pages 43-49

  7. 3D Endothelial Cell Migration

    • Kathryn A. Jacobs, Julie Gavard
    Pages 51-58

  8. Transmigration of Leukocytes Across Epithelial Monolayers

    • Penny E. Morton, Maddy Parsons
    Pages 59-70

  9. Evaluation of Tumor Cell Invasiveness In Vivo: The Chick Chorioallantoic Membrane Assay

    • Selma Maacha, Simon Saule
    Pages 71-77

  10. Analysis of Invasion Dynamics of Matrix-Embedded Cells in a Multisample Format

    • Marleen Van Troys, Paola Masuzzo, Lynn Huyck, Karima Bakkali, Davy Waterschoot, Lennart Martens et al.
    Pages 79-117

  11. Using Systems Microscopy to Understand the Emergence of Cell Migration from Cell Organization

    • Staffan Strömblad, John G. Lock
    Pages 119-134

  12. Neuronal Precursor Migration in Ex Vivo Brain Slice Culture

    • Fu-Sheng Chou, Pei-Shan Wang
    Pages 135-143

  13. In Vitro Models to Analyze the Migration of MGE-Derived Interneurons

    • Claire Leclech, Christine Métin
    Pages 145-161

  14. Cell Migration in Tissues: Explant Culture and Live Imaging

    • Ralitza Staneva, Jorge Barbazan, Anthony Simon, Danijela Matic Vignjevic, Denis Krndija
    Pages 163-173

  15. Intravital Imaging of Tumor Cell Motility in the Tumor Microenvironment Context

    • Battuya Bayarmagnai, Louisiane Perrin, Kamyar Esmaeili Pourfarhangi, Bojana Gligorijevic
    Pages 175-193

  16. Using the Zebrafish Embryo to Dissect the Early Steps of the Metastasis Cascade

    • Gautier Follain, Naël Osmani, Cédric Fuchs, Guillaume Allio, Sébastien Harlepp, Jacky G. Goetz
    Pages 195-211

  17. Analysis of In Vivo Cell Migration in Mosaic Zebrafish Embryos

    • Arthur Boutillon, Florence A. Giger, Nicolas B. David
    Pages 213-226

  18. Analysis of Cell Shape and Cell Migration of Drosophila Macrophages In Vivo

    • Marike Rüder, Benedikt M. Nagel, Sven Bogdan
    Pages 227-238

  19. Migration of Q Cells in Caenorhabditis elegans

    • Yongping Chai, Zhiwen Zhu, Guangshuo Ou
    Pages 239-255

  20. Imaging the Molecular Machines That Power Cell Migration

    • Anika Steffen, Frieda Kage, Klemens Rottner
    Pages 257-277
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Keywords

About this book

This volume covers various assays and techniques that have been developed to study and characterize the cell migration in vitro, ex vivo, and in vivo. The chapters in this book present readers with the latest protocols to observe, quantify, and control cell migration. Some of the topics explored in this book are: migration in confined environments, microfluidic devices, optogenetics, chemotaxis, electrotaxis, detection of migrasomes, migration of Q cells in Caenorhabditis elegans, of Drosophila macrophages, optogenetics of cell migration, intravital imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and comprehensive, Cell Migration: Methods and Protocols is a valuable resource for anyone interested in learning more about this expanding field.

Reviews

“This book presents several techniques to study cells migration in vivo, in vitro, ex vivo and with different model systems to dissect many of the biochemical and biophysical properties (at both molecular and cellular levels) involved in the dynamics of migration and cell-to-cell communication. … the editor of this book did a great job in collecting contributions where several animal models are presented.” (Manuela Monti, European Journal of Histochemistry, Vol. 63 (01), 2019)​

Editors and Affiliations

  • Laboratory of Biochemistry, Ecole Polytechnique, Palaiseau, France

    Alexis Gautreau

Bibliographic Information

  • Book Title: Cell Migration

  • Book Subtitle: Methods and Protocols

  • Editors: Alexis Gautreau

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7701-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7700-0Published: 11 March 2018

  • Softcover ISBN: 978-1-4939-9261-4Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7701-7Published: 10 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 405

  • Number of Illustrations: 10 b/w illustrations, 88 illustrations in colour

  • Topics: Cell Biology

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