Skip to main content
SpringerLink
Log in
Book cover

Nitric Oxide

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Alexander Mengel

    1. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Christian Lindermayr

    1. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1747)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 149.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 199.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. NO Treatment and Detection

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. A Simple and Useful Method to Apply Exogenous NO Gas to Plant Systems: Bell Pepper Fruits as a Model

      • José M. Palma, Carmelo Ruiz, Francisco J. Corpas
      Pages 3-11

    3. Measurements of Intra-oocyte Nitric Oxide Concentration Using Nitric Oxide Selective Electrode

      • Sana N. Khan, Roohi Jeelani, Husam M. Abu-Soud
      Pages 13-21

    4. Real-Time Imaging of Nitric Oxide Signals in Individual Cells Using geNOps

      • Emrah Eroglu, Helmut Bischof, Suphachai Charoensin, Markus Waldeck-Weiermaier, Wolfgang F. Graier, Roland Malli
      Pages 23-34

    5. Detection of Nitric Oxide by Membrane Inlet Mass Spectrometry

      • John M. Goodwin, Carol A. Chrestensen, Ellen W. Moomaw
      Pages 35-47

    6. Quantum Cascade Lasers-Based Detection of Nitric Oxide

      • Gracia Montilla-Bascón, Julien Mandon, Frans J. M. Harren, Luis A. J. Mur, Simona M. Cristescu, Elena Prats
      Pages 49-57

    7. Detection of Nitric Oxide via Electronic Paramagnetic Resonance in Mollusks

      • Paula Mariela González, Susana Puntarulo
      Pages 59-69

  3. NO Modifications

    1. Front Matter

      Pages 71-71
      Download chapter PDF

    2. Identification of S-Nitrosylated and Reversibly Oxidized Proteins by Fluorescence Switch and Complementary Techniques

      • Alicia Izquierdo-Álvarez, Daniel Tello, J. Daniel Cabrera-García, Antonio Martínez-Ruiz
      Pages 73-87

    3. A Proteomics Workflow for Dual Labeling Biotin Switch Assay to Detect and Quantify Protein S-Nitroylation

      • Heaseung Sophia Chung, Christopher I. Murray, Jennifer E. Van Eyk
      Pages 89-101

    4. Surface Plasmon Resonance Spectroscopy for Detection of S-Nitrosylated Proteins

      • Lili Zhang, Pengpeng Shang, Changbao Chen, Jie Zhou, Shuhua Zhu
      Pages 103-111

    5. Measurement of S -Nitrosoglutathione in Plasma by Liquid Chromatography–Tandem Mass Spectrometry

      • Dimitrios Tsikas, Erik Hanff
      Pages 113-129

    6. Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Technique

      • Sébastien Aimé, Siham Hichami, David Wendehenne, Olivier Lamotte
      Pages 131-141

    7. Direct Measurement of S-Nitrosothiols with an Orbitrap Fusion Mass Spectrometer: S-Nitrosoglutathione Reductase as a Model Protein

      • Damian Guerra, Ian Truebridge, Stephen J. Eyles, Patrick Treffon, Elizabeth Vierling
      Pages 143-160

    8. Identification of Tyrosine and Nitrotyrosine with a Mixed-Mode Solid-Phase Extraction Cleanup Followed by Liquid Chromatography–Electrospray Time-of-Flight Mass Spectrometry in Plants

      • Mounira Chaki, Beatriz Sánchez-Calvo, Alfonso Carreras, Raquel Valderrama, Juan C. Begara-Morales, Francisco J. Corpas et al.
      Pages 161-169

    9. Electrophoretic Detection and Confocal Microscopic Imaging of Tyrosine Nitrated Proteins in Plant Tissue

      • Dhara Arora, Neha Singh, Satish C. Bhatla
      Pages 171-182

    10. Identification of NO-Sensitive Cysteine Residues Using Cysteine Mutants of Recombinant Proteins

      • Azam Shekariesfahlan, Christian Lindermayr
      Pages 183-203

    11. Detection of S-Nitrosated Nuclear Proteins in Pathogen-Treated Arabidopsis Cell Cultures Using Biotin Switch Technique

      • Azam Shekariesfahlan, Christian Lindermayr
      Pages 205-221

    12. Nitric Oxide Analyzer Quantification of Plant S-Nitrosothiols

      • Adil Hussain, Byung-Wook Yun, Gary J. Loake
      Pages 223-230
Back to top

Keywords

About this book

This volume explores three main aspects of nitric oxide (NO) research: NO treatment and detection, NO modifications, and NO detoxification. The book also covers methods used to study human/animal and plant nitric oxide. The chapters are divided into three parts: part one looks at NO treatments using gaseous nitric oxide and detection using a NO-sensitive electrode, electron spin resonance, and fluorescence-based NO-sensor proteins. Part two talks about various techniques used to detect and identify NO-dependent modifications, such as biotin-switch assay and quantification of s-nitrosated proteins. Part three focuses on the study of s-nitrosothiol homeostasis and denitrosation activities. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting edge and authoritative, Nitric Oxide: Methods and Protocols is a valuable resource for anyone interested in learning more about this evolving field.



Editors and Affiliations

  • Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Alexander Mengel, Christian Lindermayr

Bibliographic Information

  • Book Title: Nitric Oxide

  • Book Subtitle: Methods and Protocols

  • Editors: Alexander Mengel, Christian Lindermayr

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7695-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7694-2Published: 30 March 2018

  • Softcover ISBN: 978-1-4939-9260-7Published: 10 December 2019

  • eBook ISBN: 978-1-4939-7695-9Published: 30 March 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 302

  • Number of Illustrations: 22 b/w illustrations, 45 illustrations in colour

  • Topics: Biochemistry, general

Publish with us

Policies and ethics

Back to top

Search

Navigation