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Hypoxia

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. L. Eric Huang

    1. Department of Neurosurgery, Clinical Neurosciences Center, University of Utah, Salt Lake City, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1742)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Genetic Knockdown and Pharmacologic Inhibition of Hypoxia-Inducible Factor (HIF) Hydroxylases

    • Christina Pickel, Cormac T. Taylor, Carsten C. Scholz
    Pages 1-14

  3. Kinetic Analysis of HIF Prolyl Hydroxylases

    • Peppi Koivunen, Johanna Myllyharju
    Pages 15-25

  4. Mass Spectrometry and Bioinformatic Analysis of Hydroxylation-Dependent Protein-Protein Interactions

    • Javier Rodriguez, Alex von Kriegsheim
    Pages 27-36

  5. Acquisition of Temporal HIF Transcriptional Activity Using a Secreted Luciferase Assay

    • Miguel A. S. Cavadas, Cormac T. Taylor, Alex Cheong
    Pages 37-44

  6. Fluorescence Lifetime Imaging Microscopy (FLIM) as a Tool to Investigate Hypoxia-Induced Protein-Protein Interaction in Living Cells

    • Vera Schützhold, Joachim Fandrey, Katrin Prost-Fingerle
    Pages 45-53

  7. Transcriptional Profiling Using RNA-Seq to Study Hypoxia-Mediated Gene Regulation

    • Surendra K. Shukla, Ryan J. King, Pankaj K. Singh
    Pages 55-66

  8. Chromatin Immunoprecipitation of HIF-α in Breast Tumor Cells Using Wild Type and Loss of Function Models

    • Danielle L. Brooks, Tiffany N. Seagroves
    Pages 67-79

  9. Evaluating the Metabolic Impact of Hypoxia on Pancreatic Cancer Cells

    • Divya Murthy, Enza Vernucci, Gennifer Goode, Jaime Abrego, Pankaj K. Singh
    Pages 81-93

  10. Hypoxia-Induced Metabolomic Alterations in Pancreatic Cancer Cells

    • Venugopal Gunda, Sushil Kumar, Aneesha Dasgupta, Pankaj K. Singh
    Pages 95-105

  11. Hypoxia-Mediated In Vivo Tumor Glucose Uptake Measurement and Analysis

    • Surendra K. Shukla, Scott E. Mulder, Pankaj K. Singh
    Pages 107-113

  12. Measurement of Sensory Nerve Activity from the Carotid Body

    • Ying-Jie Peng, Nanduri R. Prabhakar
    Pages 115-124

  13. Monitoring Functional Responses to Hypoxia in Single Carotid Body Cells

    • Ana María Muñoz-Cabello, Hortensia Torres-Torrelo, Ignacio Arias-Mayenco, Patricia Ortega-Sáenz, José López-Barneo
    Pages 125-137

  14. Testing Acute Oxygen Sensing in Genetically Modified Mice: Plethysmography and Amperometry

    • Patricia Ortega-Sáenz, Candela Caballero, Lin Gao, José López-Barneo
    Pages 139-153

  15. Immunohistochemistry of the Carotid Body

    • Jayasri Nanduri, Nanduri R. Prabhakar
    Pages 155-166

  16. Hypoxia Signaling and Placental Adaptations

    • Damayanti Chakraborty, Regan L. Scott, Michael J. Soares
    Pages 167-183

  17. Evaluation of Erythrocyte Changes After Normoxic Return from Hypoxia

    • Jihyun Song, Josef T. Prchal
    Pages 185-194

  18. Hypoxic Treatment of Zebrafish Embryos and Larvae

    • Hiroyasu Kamei, Cunming Duan
    Pages 195-203

  19. Microinjection of Antisense Morpholinos, CRISPR/Cas9 RNP, and RNA/DNA into Zebrafish Embryos

    • Yi Xin, Cunming Duan
    Pages 205-211

  20. Western Blot Analysis of C. elegans Proteins

    • Dae-Eun Jeong, Yujin Lee, Seung-Jae V. Lee
    Pages 213-225
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Keywords

About this book

This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases. Chapters guide the reader through the application of hypoxic conditions, to the techniques of molecular biology, biochemical, cell biology, genomic,bioinformatic, metabolic, and animal studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Hypoxia: Methods and Protocols aims to provide a valuable set of tools that can be used to study hypoxia and beyond.

Editors and Affiliations

  • Department of Neurosurgery, Clinical Neurosciences Center, University of Utah, Salt Lake City, USA

    L. Eric Huang

Bibliographic Information

  • Book Title: Hypoxia

  • Book Subtitle: Methods and Protocols

  • Editors: L. Eric Huang

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7665-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7664-5Published: 13 January 2018

  • Softcover ISBN: 978-1-4939-8538-8Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7665-2Published: 11 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 302

  • Number of Illustrations: 10 b/w illustrations, 63 illustrations in colour

  • Topics: Cell Biology

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