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Extracellular RNA

Methods and Protocols

Editors:

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1740)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
  2. Extracellular RNAs: A New Awareness of Old Perspectives

    • Noah Sadik, Lilian Cruz, Alessandra Gurtner, Rodosthenis S. Rodosthenous, Sophie A. Dusoswa, Olivia Ziegler et al.
    Pages 1-15
  3. Overview of Protocols for Studying Extracellular RNA and Extracellular Vesicles

    • Julia Small, Sabrina Roy, Roger Alexander, Leonora Balaj
    Pages 17-21
  4. Extracellular RNA Isolation from Cell Culture Supernatant

    • Sawen Bakr, Bridget Simonson, Kirsty M. Danielson, Saumya Das
    Pages 23-34
  5. Use of a Hollow Fiber Bioreactor to Collect Extracellular Vesicles from Cells in Culture

    • Irene K. Yan, Neha Shukla, David A. Borrelli, Tushar Patel
    Pages 35-41
  6. Isolation of Extracellular RNA from Serum/Plasma

    • Justyna Filant, Parham Nejad, Anu Paul, Bridget Simonson, Srimeenakshi Srinivasan, Xuan Zhang et al.
    Pages 43-57
  7. Isolation of Extracellular RNA from Bile

    • Irene K. Yan, Valentin X. Berdah, Tushar Patel
    Pages 59-67
  8. Enrichment of Extracellular Vesicle Subpopulations Via Affinity Chromatography

    • Michelle E. Hung, Stephen B. Lenzini, Devin M. Stranford, Joshua N. Leonard
    Pages 109-124
  9. Detection and Analysis of Non-vesicular Extracellular RNA

    • Juan Pablo Tosar, Alfonso Cayota
    Pages 125-137
  10. Isolation of Plasma Lipoproteins as a Source of Extracellular RNA

    • Kang Li, David K. Wong, Fu Sang Luk, Roy Y. Kim, Robert L. Raffai
    Pages 139-153
  11. Droplet Digital PCR for Quantitation of Extracellular RNA

    • Irene K. Yan, Rishabh Lohray, Tushar Patel
    Pages 155-162
  12. Preparation of Small RNA NGS Libraries from Biofluids

    • Alton Etheridge, Kai Wang, David Baxter, David Galas
    Pages 163-175
  13. Loading of Extracellular Vesicles with Hydrophobically Modified siRNAs

    • Marie-Cecile Didiot, Reka A. Haraszti, Neil Aronin, Anastasia Khvorova
    Pages 199-214
  14. Back Matter

    Pages 215-216

About this book

This volume provides a collection of methods and protocols detailing extracellular RNA and hopes to be a useful resource for those interested in this exciting and emerging field. Chapters are divided into four sections covering an overview of the field, approaches for the isolation of exRNA, protocols for detection and quantitation of exRNA, and approaches that can be useful for studies to evaluate potential therapeutic applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Extracellular RNA: Methods and Protocols aims to ensure successful results in the further study of this vital field. 

Editors and Affiliations

  • Department of Transplantation, Mayo Clinic, Jacksonville, USA

    Tushar Patel

Bibliographic Information

  • Book Title: Extracellular RNA

  • Book Subtitle: Methods and Protocols

  • Editors: Tushar Patel

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7652-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7651-5Published: 01 February 2018

  • Softcover ISBN: 978-1-4939-8535-7Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7652-2Published: 30 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 216

  • Number of Illustrations: 13 b/w illustrations, 30 illustrations in colour

  • Topics: Human Genetics

Buy it now

Buying options

eBook USD 69.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 89.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 119.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access