Overview
- Editors:
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Georgios A. Theodoridis
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Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece
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Helen G. Gika
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School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece
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Ian D. Wilson
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Department of Surgery and Cancer, Imperial College London, London, United Kingdom
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
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Table of contents (19 protocols)
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Fundamentals
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- Helen G. Gika, Georgios A. Theodoridis, Ian D. Wilson
Pages 3-13
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- Olga Begou, Helen G. Gika, Georgios A. Theodoridis, Ian D. Wilson
Pages 15-26
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- Samantha Riccadonna, Pietro Franceschi
Pages 27-39
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Methods
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- Christina Virgiliou, Helen G. Gika, Georgios A. Theodoridis
Pages 65-81
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- Dimitra Benaki, Emmanuel Mikros
Pages 117-131
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- Matthaios-Emmanouil P. Papadimitropoulos, Catherine G. Vasilopoulou, Christoniki Maga-Nteve, Maria I. Klapa
Pages 133-147
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- Olga Deda, Helen G. Gika, Georgios A. Theodoridis
Pages 149-157
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- Petr Hušek, Zdeněk Švagera, Dagmar Hanzlíková, Iva Karlínová, Lucie Řimnáčová, Helena Zahradníčková et al.
Pages 159-181
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Plant/Food Applications
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Front Matter
Pages 193-193
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- Jan Schripsema, Denise Dagnino
Pages 195-202
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- Evangelia Ralli, Maria Amargianitaki, Efi Manolopoulou, Maria Misiak, Georgios Markakis, Sofia Tachtalidou et al.
Pages 203-211
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- Panagiotis Arapitsas, Fulvio Mattivi
Pages 225-235
About this book
This volume explores the different approaches and techniques used by researchers to study the recent challenges and developments in metabolic profiling. This book is divided into IV parts. Part I contains chapters that highlight basic concepts, such as experimental design, data treatment, metabolite identification, and harmonization. Part II describes experimental protocols for both targeted and untargeted metabolomics covering the basic analytical technologies: LC-MS, GC-MS, NMR and CE-MS. In addition the protocols describe methods for the study of tissues, feces, blood and other types of biological samples as well as the application of chemical derivatization for GC-MS. Parts III and IV present the use of metabolomics in the study of food, plants and the life sciences, with examples from the quest for the discovery of disease biomarkers, physical exercise omics and metabolic profiling of food, fruit and wine. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Metabolic Profiling: Methods and Protocols is a valuable resource for researchers who are interested in expanding their knowledge of this rapidly developing field.
Editors and Affiliations
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Department of Chemistry, Aristotle University of Thessaloniki, Thessaloniki, Greece
Georgios A. Theodoridis
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School of Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece
Helen G. Gika
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Department of Surgery and Cancer, Imperial College London, London, United Kingdom
Ian D. Wilson
