Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 1732)
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Table of contents (37 protocols)
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Front Matter
About this book
This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.
Editors and Affiliations
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Department of Pathology CARIM School for Cardiovascular Diseases, Maastricht University, Maastricht, The Netherlands
Dietbert Neumann
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Department EMD, Inserm, U1016, Institut Cochin, CNRS, UMR8104, Université Paris Descartes, Paris, France
Benoit Viollet
Bibliographic Information
Book Title: AMPK
Book Subtitle: Methods and Protocols
Editors: Dietbert Neumann, Benoit Viollet
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-7598-3
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC 2018
Hardcover ISBN: 978-1-4939-7597-6Published: 26 February 2018
Softcover ISBN: 978-1-4939-9249-2Published: 10 December 2019
eBook ISBN: 978-1-4939-7598-3Published: 26 February 2018
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XVII, 626
Number of Illustrations: 42 b/w illustrations, 72 illustrations in colour
Topics: Protein Science