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Proteases and Cancer

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Santiago Cal

    1. Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, Oviedo, Spain

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  2. Alvaro J. Obaya

    1. Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, Oviedo, Spain

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1731)

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Table of contents (27 protocols)

  1. Front Matter

    Pages i-xv
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  2. Dissecting Degradomes: Analysis of Protease-Coding Genes

    • Ángel Álvarez-Eguiluz, Ander Díaz-Navarro, Xose S. Puente
    Pages 1-13

  3. Identification of Protease Cleavage Sites and Substrates in Cancer by Carboxy-TAILS (C-TAILS)

    • Nestor Solis, Christopher M. Overall
    Pages 15-28

  4. Identification of Proteolytic Cleavage Sites of EphA2 by Membrane Type 1 Matrix Metalloproteinase on the Surface of Cancer Cells

    • Keiji Kikuchi, Hiroko Kozuka-Hata, Masaaki Oyama, Motoharu Seiki, Naohiko Koshikawa
    Pages 29-37

  5. Biotin-Chasing Assay to Evaluate uPAR Stability and Cleavage on the Surface of Cells

    • Vladimir Leksa, Herbert B. Schiller, Hannes Stockinger
    Pages 39-47

  6. Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease

    • Pedro M. Quirós
    Pages 49-56

  7. A Simple Cell-Based Assay for the Detection of Surface Protein Shedding by Rhomboid Proteases

    • Angela Moncada-Pazos, Adam Graham Grieve
    Pages 57-64

  8. Functional Production of Catalytic Domains of Human MMPs in Escherichia coli Periplasm

    • Dong Hyun Nam, Ki Baek Lee, Xin Ge
    Pages 65-72

  9. Autophagy and Proteases: Basic Study of the Autophagic Flux by Western Blot

    • Álvaro F. Fernández
    Pages 73-81

  10. Gel-Based Gelatin Zymography to Examine Matrix Metalloproteinase Activity in Cell Culture

    • Aastha Chhabra, Vibha Rani
    Pages 83-96

  11. Analysis of Enzymatic Activity of Matrix Metalloproteinase (MMP) by Collagen Zymography in Melanoma

    • Vijay Walia, Yardena Samuels
    Pages 97-106

  12. Measurement of Protease Activities Using Fluorogenic Substrates

    • Salvatore Santamaria, Hideaki Nagase
    Pages 107-122

  13. Targeting the Expression of Cathepsin B Using CRISPR/Cas9 System in Mammalian Cancer Cells

    • Manu Gnanamony, Christopher S. Gondi
    Pages 123-131

  14. Assessing the Influence of a Protease in Cell Migration Using the Barrier-Migration Assay

    • Tania Fontanil, Yamina Mohamedi, Santiago Cal, Álvaro J. Obaya
    Pages 133-143

  15. Analysis of Invasive Activity of CAF Spheroids into Three Dimensional (3D) Collagen Matrices

    • María Ángeles Villaronga, Saúl Álvarez Teijeiro, Francisco Hermida-Prado, Marta Garzón-Arango, Victoria Sanz-Moreno, Juana María García-Pedrero
    Pages 145-154

  16. Beyond the Proteolytic Activity: Examining the Functional Relevance of the Ancillary Domains Using Tri-Dimensional (3D) Spheroid Invasion Assay

    • Erico Tosoni Costa, Anamaria Aranha Camargo
    Pages 155-168

  17. Analyzing the Type II Transmembrane Serine Protease Hepsin-Dependent Basement Membrane Remodeling in 3D Cell Culture

    • Shishir M. Pant, Denis Belitskin, Hanna Ala-Hongisto, Juha Klefström, Topi A. Tervonen
    Pages 169-178

  18. Evaluation of Tumor Vasculature Using a Syngeneic Tumor Model in Wild-Type and Genetically Modified Mice

    • Francisco Javier Rodríguez-Baena, Silvia Redondo-García, María del Carmen Plaza-Calonge, Rubén Fernández-Rodríguez, Juan Carlos Rodríguez-Manzaneque
    Pages 179-192

  19. 3D Image Analysis of the Microvasculature in Healthy and Diseased Tissues

    • Álvaro Sahún-Español, Cristina Clemente, Alicia G. Arroyo
    Pages 193-212

  20. Human Tumor Tissue-Based 3D In Vitro Invasion Assays

    • Pirjo Åström, Ritva Heljasvaara, Pia Nyberg, Ahmed Al-Samadi, Tuula Salo
    Pages 213-221
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Keywords

About this book

This volume explores the role of proteases in various catalytic classes and cellular processes associated with cancer. The chapters in this book cover topics such as encoding proteolytic enzymes, cleavage sites of proteases, functional catalytic domains of MMPs, zymography,  angiogenesis and lymphangiogenesis, genetic and epigenetic alterations of metallopeptidases, and methods to target metalloproteinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting edge, Proteases and Cancer: Methods and Protocols is a valuable resource for clinical researchers across numerous fields such as molecular and cellular biology, oncology, biochemistry, and chemistry. This book is also useful for PhD students in the various disciples of health sciences.



Editors and Affiliations

  • Instituto Universitario de Oncología (IUOPA), Universidad de Oviedo, Oviedo, Spain

    Santiago Cal, Alvaro J. Obaya

Bibliographic Information

  • Book Title: Proteases and Cancer

  • Book Subtitle: Methods and Protocols

  • Editors: Santiago Cal, Alvaro J. Obaya

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7595-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7594-5Published: 10 January 2018

  • Softcover ISBN: 978-1-4939-8531-9Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7595-2Published: 09 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 352

  • Number of Illustrations: 17 b/w illustrations, 41 illustrations in colour

  • Topics: Cancer Research

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