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Clinical Metabolomics

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Martin Giera

    1. Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands

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  • Includes cutting-edge methods and protocols for the use of clinical metabolomics
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1730)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Clinical Metabolomics and Lipidomics

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Metabolomics as a Tool to Understand Pathophysiological Processes

      • Julijana Ivanisevic, Aurelien Thomas
      Pages 3-28

    3. Metabolomics in Immunology Research

      • Bart Everts
      Pages 29-42

  3. LC-MS-Based Metabolomics

    1. Front Matter

      Pages 43-43
      Download chapter PDF

    2. LC-MS-Based Metabolomics of Biofluids Using All-Ion Fragmentation (AIF) Acquisition

      • Romanas Chaleckis, Shama Naz, Isabel Meister, Craig E. Wheelock
      Pages 45-58

    3. Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis

      • Jesmond Dalli, Romain A. Colas, Mary E. Walker, Charles N. Serhan
      Pages 59-72

    4. UHPSFC/ESI-MS Analysis of Lipids

      • Miroslav Lísa, Michal Holčapek
      Pages 73-82

    5. LC-MS/MS Analysis of Lipid Oxidation Products in Blood and Tissue Samples

      • Yiu Yiu Lee, Jetty Chung-Yung Lee
      Pages 83-92

    6. Serum Testosterone by Liquid Chromatography Tandem Mass Spectrometry for Routine Clinical Diagnostics

      • Lennart J. van Winden, Olaf van Tellingen, Huub H. van Rossum
      Pages 93-102

    7. LC-MS/MS Analysis of Bile Acids

      • Sabrina Krautbauer, Gerhard Liebisch
      Pages 103-110

    8. LC-MS/MS Analysis of Triglycerides in Blood-Derived Samples

      • Madlen Reinicke, Susen Becker, Uta Ceglarek
      Pages 111-121

    9. LC-MS/MS Analysis of the Epoxides and Diols Derived from the Endocannabinoid Arachidonoyl Ethanolamide

      • Amy A. Rand, Patrick O. Helmer, Bora Inceoglu, Bruce D. Hammock, Christophe Morisseau
      Pages 123-133

    10. Sphingolipid Analysis in Clinical Research

      • Bo Burla, Sneha Muralidharan, Markus R. Wenk, Federico Torta
      Pages 135-162

    11. Shotgun Lipidomics Approach for Clinical Samples

      • Lars F. Eggers, Dominik Schwudke
      Pages 163-174

    12. Establishing and Performing Targeted Multi-residue Analysis for Lipid Mediators and Fatty Acids in Small Clinical Plasma Samples

      • Theresa L. Pedersen, John W. Newman
      Pages 175-212

    13. Chemical Isotope Labeling LC-MS for Human Blood Metabolome Analysis

      • Wei Han, Liang Li
      Pages 213-225

    14. Direct Infusion-Tandem Mass Spectrometry (DI-MS/MS) Analysis of Complex Lipids in Human Plasma and Serum Using the Lipidyzer™ Platform

      • Baljit K. Ubhi
      Pages 227-236

  4. GC-MS-Based Metabolomics

    1. Front Matter

      Pages 237-237
      Download chapter PDF

    2. Exploratory GC/MS-Based Metabolomics of Body Fluids

      • Carole Migné, Stéphanie Durand, Estelle Pujos-Guillot
      Pages 239-246
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Keywords

About this book

This detailed volume presents a comprehensive compendium of clinical metabolomics protocols covering LC-MS, GC-MS, CE-MS, and NMR-based clinical metabolomics as well as bioinformatics and study design considerations. The methodologies explored here form the core of several very promising initiatives evolving around personalized health care and precision medicine, which can be seen as complimentary to the field of clinical chemistry and aid the aforementioned field with novel disease markers and diagnostic patterns. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Clinical Metabolomics: Methods and Protocols aims to serve as the basis for successful communication between scientists from several fields, including chemists, biologist, bioinformaticians, and clinicians, ultimately leading to effective study design and completion.

Editors and Affiliations

  • Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands

    Martin Giera

Bibliographic Information

  • Book Title: Clinical Metabolomics

  • Book Subtitle: Methods and Protocols

  • Editors: Martin Giera

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7592-1

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7591-4Published: 24 January 2018

  • Softcover ISBN: 978-1-4939-8530-2Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7592-1Published: 23 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 389

  • Number of Illustrations: 17 b/w illustrations, 22 illustrations in colour

  • Topics: Biochemistry, general

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