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The Retinoblastoma Protein

  • Book
  • © 2018

Overview

Editors:
  1. Pedro G. Santiago-Cardona

    1. Biochemistry and Cancer Biology Divisions Basic Science Department, Ponce Health Sciences University, Ponce, Puerto Rico

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1726)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Characterization of RB1 Deletions in Interphase and Metaphase by Molecular Cytogenetics Exemplified in Chronic Lymphatic Leukemia

    • Thomas Liehr
    Pages 1-6

  3. Detection of RB1 Gene Copy Number Variations Using a Multiplex Ligation-Dependent Probe Amplification Method

    • Nejat Dalay
    Pages 7-18

  4. A Fluorescent Quantitative Multiplex PCR Method to Detect Copy Number Changes in the RB1 Gene

    • Chitra Kannabiran
    Pages 19-28

  5. Using Methylation-Specific PCR to Study RB1 Promoter Hypermethylation

    • Thaís M. McCormick, Maria Da Glória Da C. Carvalho
    Pages 29-34

  6. Detection of Aberrant DNA Methylation Patterns in the RB1 Gene

    • Sumadi Lukman Anwar, Ulrich Lehmann
    Pages 35-47

  7. Detection of Retinoblastoma Protein Phosphorylation by Immunoblot Analysis

    • Pedro G. Santiago-Cardona, Jaileene Pérez-Morales, Jonathan González-Flores
    Pages 49-64

  8. Immunohistochemical Detection of the Retinoblastoma Protein

    • Charles A. Ishak, Matthew J. Cecchini, Christopher J. Howlett, Frederick A. Dick
    Pages 65-75

  9. Immunohistochemical Detection of Retinoblastoma Protein Phosphorylation in Human Tumor Samples

    • Jaileene Pérez-Morales, Angel Núñez-Marrero, Pedro G. Santiago-Cardona
    Pages 77-84

  10. Detection of CCND1 Locus Amplification by Fluorescence In Situ Hybridization

    • Margit Balázs, Viktória Koroknai, István Szász, Szilvia Ecsedi
    Pages 85-100

  11. Detection of CCND1 Gene Copy Number Variations Using Multiplex Ligation-Dependent Probe Amplification and Fluorescence In Situ Hybridization Methods

    • Akishi Ooi, Takeru Oyama
    Pages 101-109

  12. Detection of p16 Promoter Hypermethylation by Methylation-Specific PCR

    • Javed Hussain Choudhury, Raima Das, Shaheen Laskar, Sharbadeb Kundu, Manish Kumar, Partha Pratim Das et al.
    Pages 111-122

  13. Immunohistochemical Detection of p16 in Clinical Samples

    • Georgia Karpathiou, Michel Peoc’h
    Pages 123-142

  14. Detection of E2F-DNA Complexes Using Chromatin Immunoprecipitation Assays

    • Miyoung Lee, Lorraine J. Gudas, Harold I. Saavedra
    Pages 143-151

  15. Detection of E2F-Induced Transcriptional Activity Using a Dual Luciferase Reporter Assay

    • Ainhoa Iglesias-Ara, Nerea Osinalde, Ana M. Zubiaga
    Pages 153-166

  16. Detection of HPV E6/E7 mRNA in Clinical Samples Using RNA In Situ Hybridization

    • Manishkumar Pandey, Priyanka G. Bhosale, Manoj B. Mahimkar
    Pages 167-175

  17. CRISPR/Cas9-Mediated Knockout of Rb1 in Xenopus tropicalis

    • Thomas Naert, Kris Vleminckx
    Pages 177-193

  18. Back Matter

    Pages 195-200
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Keywords

About this book

This volume covers the mechanisms of pRb inactivation detailing repressive mechanisms commonly associated to cancer, and representative of the experimentally relevant tests used in the establishment of cancer diagnosis and prognosis. Chapters contain protocols and in-depth discussions for commonly used experimental approaches to assess the status and function of components of the pRb pathway, including pRb itself, in cell lines and biological samples.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, The Retinoblastoma Protein aims to serve as a guide to assist molecular cancer biologists in their search for understanding of the molecular functions of this preeminent tumorsuppressor.

Editors and Affiliations

  • Biochemistry and Cancer Biology Divisions Basic Science Department, Ponce Health Sciences University, Ponce, Puerto Rico

    Pedro G. Santiago-Cardona

Bibliographic Information

  • Book Title: The Retinoblastoma Protein

  • Editors: Pedro G. Santiago-Cardona

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7565-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7564-8Published: 22 February 2018

  • Softcover ISBN: 978-1-4939-8522-7Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7565-5Published: 21 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 200

  • Number of Illustrations: 6 b/w illustrations, 32 illustrations in colour

  • Topics: Cancer Research

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