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Circular RNAs

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Christoph Dieterich

    1. Department of Internal Medicine III Klaus Tschira Institute for Integrative Computational Cardiology, University Hospital Heidelberg, Heidelberg, Germany

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  2. Argyris Papantonis

    1. Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany

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    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1724)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
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  2. Detection and Reconstruction of Circular RNAs from Transcriptomic Data

    • Yi Zheng, Fangqing Zhao
    Pages 1-8

  3. Deep Computational Circular RNA Analytics from RNA-seq Data

    • Tobias Jakobi, Christoph Dieterich
    Pages 9-25

  4. Genome-Wide circRNA Profiling from RNA-seq Data

    • Daphne A. Cooper, Mariela Cortés-López, Pedro Miura
    Pages 27-41

  5. Analysis of Circular RNAs Using the Web Tool CircInteractome

    • Amaresh C. Panda, Dawood B. Dudekula, Kotb Abdelmohsen, Myriam Gorospe
    Pages 43-56

  6. Characterization and Validation of Circular RNA and Their Host Gene mRNA Expression Using PCR

    • Andreas W. Heumüller, Jes-Niels Boeckel
    Pages 57-67

  7. Detecting Circular RNAs by RNA Fluorescence In Situ Hybridization

    • Anne Zirkel, Argyris Papantonis
    Pages 69-75

  8. Single-Molecule Fluorescence In Situ Hybridization (FISH) of Circular RNA CDR1as

    • Christine Kocks, Anastasiya Boltengagen, Monika Piwecka, Agnieszka Rybak-Wolf, Nikolaus Rajewsky
    Pages 77-96

  9. A Highly Efficient Strategy for Overexpressing circRNAs

    • Dawei Liu, Vanessa Conn, Gregory J. Goodall, Simon J. Conn
    Pages 97-105

  10. Constructing GFP-Based Reporter to Study Back Splicing and Translation of Circular RNA

    • Yun Yang, Zefeng Wang
    Pages 107-118

  11. Northern Blot Analysis of Circular RNAs

    • Tim Schneider, Silke Schreiner, Christian Preußer, Albrecht Bindereif, Oliver Rossbach
    Pages 119-133

  12. Nonradioactive Northern Blot of circRNAs

    • Xiaolin Wang, Ge Shan
    Pages 135-141

  13. Characterization of Circular RNA Concatemers

    • Thomas B. Hansen
    Pages 143-157

  14. Characterization of Circular RNAs (circRNA) Associated with the Translation Machinery

    • Deniz Bartsch, Anne Zirkel, Leo Kurian
    Pages 159-166

  15. Synthesis and Engineering of Circular RNAs

    • Sonja Petkovic, Sabine Müller
    Pages 167-180

  16. Preparation of Circular RNA In Vitro

    • Naoko Abe, Ayumi Kodama, Hiroshi Abe
    Pages 181-192

  17. Discovering circRNA-microRNA Interactions from CLIP-Seq Data

    • Xiao-Qin Zhang, Jian-Hua Yang
    Pages 193-207

  18. Identification of circRNAs for miRNA Targets by Argonaute2 RNA Immunoprecipitation and Luciferase Screening Assays

    • Yan Li, Bing Chen, Shenglin Huang
    Pages 209-218

  19. Back Matter

    Pages 219-220
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Keywords

About this book

This volume provides established approaches for identifying, characterizing, and manipulating circRNAs in vitro, in vivo, and in silico.  Chapters highlight the breakthroughs and the challenges in this new field of research. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Circular RNAs: Methods and Protocols aims to useful and informative for further study into this vital field. 

Editors and Affiliations

  • Department of Internal Medicine III Klaus Tschira Institute for Integrative Computational Cardiology, University Hospital Heidelberg, Heidelberg, Germany

    Christoph Dieterich

  • Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany

    Argyris Papantonis

Bibliographic Information

  • Book Title: Circular RNAs

  • Book Subtitle: Methods and Protocols

  • Editors: Christoph Dieterich, Argyris Papantonis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7562-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7561-7Published: 10 January 2018

  • Softcover ISBN: 978-1-4939-8521-0Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7562-4Published: 08 January 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIII, 220

  • Number of Illustrations: 17 b/w illustrations, 33 illustrations in colour

  • Topics: Human Genetics

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