Laser Capture Microdissection

1. Front Matter

   Pages i-xv

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2. Laser Capture Microdissection: Insights into Methods and Applications

   • Meera Mahalingam

   Pages 1-17

3. Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues

   • Rémi Longuespée, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, Edwin De Pauw

   Pages 19-31

4. Laser Microdissection Workflow for Isolating Nucleic Acids from Fixed and Frozen Tissue Samples

   • Yelena G. Golubeva, Andrew C. Warner

   Pages 33-93

5. Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE

   • DaRue A. Prieto, Gordon Whitely, Donald J. Johann Jr., Josip Blonder

   Pages 95-110

6. Next-Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens

   • Lavinia Mägel, Stephan Bartels, Ulrich Lehmann

   Pages 111-118

7. Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model

   • Nadia P. Castro, Yelena G. Golubeva

   Pages 119-137

8. Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease

   • Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio et al.

   Pages 139-154

9. Laser Capture Microdissection and Isolation of High-Quality RNA from Frozen Endometrial Tissue

   • Michele Cummings, Georgia Mappa, Nicolas M. Orsi

   Pages 155-166

10. Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions

    • Monica Molano, Suzanne M. Garland, Alyssa M. Cornall

    Pages 167-189

11. Laser Capture Microdissection and Transcriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone

    • Efrain Pacheco, Rong Hu, Scott Taylor

    Pages 191-202

12. Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain

    • Sarah A. Mauney, Tsung-Ung W. Woo, Kai C. Sonntag

    Pages 203-221

13. The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience

    • Renée Morris, Prachi Mehta

    Pages 223-233

14. Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses

    • Deborah R. Boone, Harris A. Weisz, Stacy L. Sell, Helen L. Hellmich

    Pages 235-245

15. Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High-Performance Liquid Chromatography—Mass Spectrometry

    • Luisa Aring, Simone Steinbach, Katrin Marcus, Caroline May

    Pages 247-260

16. Immuno-Guided Laser-Capture Microdissection of Glial Cells for mRNA Analysis

    • Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, David A. Laplaud

    Pages 261-271

17. Immuno-Laser-Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post-Mortem Human Brain

    • Julie E. Simpson, Stephen B. Wharton, Paul R. Heath

    Pages 273-284

18. Laser-Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR

    • Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, Eduardo Molina-Holgado

    Pages 285-318

19. Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer’s Disease Tissue Using Laser-Capture Microdissection for Downstream Proteomics

    • Eleanor Drummond, Shruti Nayak, Geoffrey Pires, Beatrix Ueberheide, Thomas Wisniewski

    Pages 319-334

20. Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post-mortem Midbrain Samples by UV-Laser Microdissection and RT-qPCR

    • Johanna Duda, Michael Fauler, Jan Gründemann, Birgit Liss

    Pages 335-360

This fully updated edition provides practical advice on how to carry out tissue-based laser microdissection successfully by using the different laser microdissection systems that are available and by applying a wide range of molecular technologies. The contents of the volume explore these techniques that have revolutionized carrying out molecular analysis on specific types of normal and diseased cells and fully utilizing the power of current molecular technologies including PCR, microarrays, next generation sequencing, and proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Laser Capture Microdissection: Methods and Protocols, Third Edition seeks to aid researchers generally and pathologists in particular in moving their studies forward with these vital techniques.

• Pathology
• Tissue-based laser microdissection
• Next generation sequencing
• Gene expression analysis
• Microarrays
• Tissue samples

• School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, United Kingdom

  Graeme I. Murray

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