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Schizosaccharomyces pombe

Methods and Protocols

  • Book
  • © 2018

Overview

Editors:
  1. Teresa L. Singleton

    1. Department of Biological Sciences, Winston-Salem State University, Winston-Salem, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1721)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Preparation of Solutions and Reagents

    • Teresa L. Singleton
    Pages 1-7

  3. Analysis of Fission Yeast Single DNA Molecules on the Megabase Scale Using DNA Combing

    • Atanas Kaykov, Paul Nurse
    Pages 9-24

  4. Chromatin Immunoprecipitation-Polymerase Chain Reaction (ChIP-PCR) Detects Methylation, Acetylation, and Ubiquitylation in S. pombe

    • Valerie Migeot, Damien Hermand
    Pages 25-34

  5. Primer Design and Inverse PCR on Yeast Display Antibody Selection Outputs

    • Fortunato Ferrara, Andrew R. M. Bradbury, Sara D’Angelo
    Pages 35-45

  6. Molecular Cloning and Characterization of Small Viral Genome in Fission Yeast

    • Ge Li, Richard Y. Zhao
    Pages 47-61

  7. Total RNA Isolation and Quantification of Specific RNAs in Fission Yeast

    • Robert Roth, Hiten D. Madhani, Jennifer F. Garcia
    Pages 63-72

  8. Analysis of Reverse Transcribed mRNA Using PCR and Polyacrylamide Gel Electrophoresis

    • Pranjal Biswas, Uddalak Majumdar, Sanjay Ghosh
    Pages 73-87

  9. The No-Nonsens SDS-PAGE

    • Lars Backman, Karina Persson
    Pages 89-94

  10. Crystallization of Recombinant α-Actinin and Related Proteins

    • Karina Persson, Lars Backman
    Pages 95-103

  11. Estimation of GFP-Nucleoporin Amount Based on Fluorescence Microscopy

    • Haruhiko Asakawa, Yasushi Hiraoka, Tokuko Haraguchi
    Pages 105-115

  12. Antibody Pull-Down Experiments in Fission Yeast

    • Qianhua Dong, Fei Li
    Pages 117-123

  13. Preparation of Cell Lysates of Fission Yeast for Immunoprecipitation

    • Akihisa Matsuyama, Atsuko Shirai, Minoru Yoshida
    Pages 125-133

  14. Wide-band Electrical Impedance Spectroscopy (EIS) Measures S. pombe Cell Growth in vivo

    • Zhen Zhu, Olivier Frey, Andreas Hierlemann
    Pages 135-153

  15. In Situ Chromatin-Binding Assay Using Epifluorescent Microscopy in S. pombe

    • Jinpu Yang, Fei Li
    Pages 155-165

  16. High-Frequency Lithium Acetate Transformation of Schizosaccharomyces pombe

    • Sudhir Kumar Rai, Angela Atwood-Moore, Henry L. Levin
    Pages 167-177

  17. Tetrad Dissection in Fission Yeast

    • Wilber Escorcia, Susan L. Forsburg
    Pages 179-187

  18. Random Spore Analysis in Fission Yeast

    • Wilber Escorcia, Susan L. Forsburg
    Pages 189-195

  19. Duplication and Transformation of the Schizosaccharomyces pombe Collection of Deletion Strains

    • Sudhir Kumar Rai, Angela Atwood-Moore, Henry L. Levin
    Pages 197-215

  20. Schizosaccharomyces pombe Biotechnological Applications in Winemaking

    • Ángel Benito, Fernando Calderón, Santiago Benito
    Pages 217-226
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Keywords

About this book

This volume presents various laboratory protocols, reviews, specific techniques and applications related to Schizosaccharomyces pombe. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Schizosaccharomyces pombe: Methods and Protocols hopes to serve as an excellent resource for faculty researchers, undergraduate student researchers, graduate researchers, government, and the medical community.

Editors and Affiliations

  • Department of Biological Sciences, Winston-Salem State University, Winston-Salem, USA

    Teresa L. Singleton

Bibliographic Information

  • Book Title: Schizosaccharomyces pombe

  • Book Subtitle: Methods and Protocols

  • Editors: Teresa L. Singleton

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7546-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7545-7Published: 08 February 2018

  • Softcover ISBN: 978-1-4939-8516-6Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7546-4Published: 08 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 244

  • Number of Illustrations: 14 b/w illustrations, 34 illustrations in colour

  • Topics: Microbiology

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