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Peptidomics

Methods and Strategies

  • Book
  • © 2018

Overview

Editors:
  1. Michael Schrader

    1. Department of Biotechnology and Bioinformatics, Weihenstephan-Tr. University of Applied Sciences, Freising, Germany

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    You can also search for this editor in PubMed   Google Scholar

  2. Lloyd Fricker

    1. Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible products
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 1719)

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Table of contents (28 protocols)

  1. Front Matter

    Pages i-xvii
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  2. Overview and Basic Techniques

    1. Front Matter

      Pages 1-1
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    2. Origins, Technological Development, and Applications of Peptidomics

      • Michael Schrader
      Pages 3-39

    3. Brain Tissue Sample Stabilization and Extraction Strategies for Neuropeptidomics

      • Elva Fridjonsdottir, Anna Nilsson, Henrik Wadensten, Per E. Andrén
      Pages 41-49

    4. Isolation of Endogenous Peptides from Cultured Cell Conditioned Media for Mass Spectrometry

      • Kazuki Sasaki, Takashi Tsuchiya, Tsukasa Osaki
      Pages 51-58

    5. Mass Spectrometric Identification of Endogenous Peptides

      • Mikel Azkargorta, Iraide Escobes, Ibon Iloro, Felix Elortza
      Pages 59-70

    6. Bioinformatics for Prohormone and Neuropeptide Discovery

      • Bruce R. Southey, Elena V. Romanova, Sandra L. Rodriguez-Zas, Jonathan V. Sweedler
      Pages 71-96

    7. Substrate Capture Assay Using Inactive Oligopeptidases to Identify Novel Peptides

      • Vanessa Rioli, Emer S. Ferro
      Pages 97-105

    8. Non-targeted Identification of d-Amino Acid-Containing Peptides Through Enzymatic Screening, Chiral Amino Acid Analysis, and LC-MS

      • Hua-Chia Tai, James W. Checco, Jonathan V. Sweedler
      Pages 107-118

  3. Quantitative Methods in Peptidomics

    1. Front Matter

      Pages 119-119
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    2. Quantitative Peptidomics: General Considerations

      • Lloyd Fricker
      Pages 121-140

    3. Quantitative Peptidomics with Isotopic and Isobaric Tags

      • Kurt Boonen, Wouter De Haes, Joris Van Houtven, Rik Verdonck, Geert Baggerman, Dirk Valkenborg et al.
      Pages 141-159

    4. Quantitative Peptidomics Using Reductive Methylation of Amines

      • Sayani Dasgupta, Leandro M. Castro, Alexandre K. Tashima, Lloyd Fricker
      Pages 161-174

    5. Metabolic Labeling to Quantify Drosophila Neuropeptides and Peptide Hormones

      • Thomas Otto Kunz, Jiangtian Chen, Megha, Christian Wegener
      Pages 175-185

    6. Data Preprocessing, Visualization, and Statistical Analyses of Nontargeted Peptidomics Data from MALDI-MS

      • Harald Tammen, Rüdiger Hess
      Pages 187-196

  4. Diverse Applied Protocols and Outlook

    1. Front Matter

      Pages 197-197
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    2. Affinity Purification of Neuropeptide Precursors from Mice Lacking Carboxypeptidase E Activity

      • Lloyd Fricker
      Pages 199-208

    3. Mass Spectrometry Based Immunopeptidomics for the Discovery of Cancer Neoantigens

      • Michal Bassani-Sternberg
      Pages 209-221

    4. Milk Peptidomics to Identify Functional Peptides and for Quality Control of Dairy Products

      • David Dallas, Søren Drud Nielsen
      Pages 223-240

    5. Neuropeptidomic Analysis of Zebrafish Brain

      • Kristien A. Van Camp, Geert Baggerman, Ronny Blust, Steven J. Husson
      Pages 241-246
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Keywords

About this book

This volume describes protocols for basic state-of-the-art approaches in the field of peptidomics. Most of these approaches are independent of the instruments used for analysis and can easily be adapted for equipment that is available in a typical proteomics facility. Chapters detail many of the basic techniques used to detect and identify peptides, methods for the relative quantitation of peptides between samples using isotopic labels or label-free approaches, and biological species as well as sample types. Written in the highly successful format of the Methods in Molecular Biology series, each chapter includes an introduction to the topic, a list of the necessary materials and reagents, reproducible step-by-step laboratory protocols, and tips on troubleshooting common problems and avoiding pitfalls.

Authoritative and practical, Peptidomics: Methods and Strategies provides useful guidance for studies in the rapidly growing field of peptidomics. 

Editors and Affiliations

  • Department of Biotechnology and Bioinformatics, Weihenstephan-Tr. University of Applied Sciences, Freising, Germany

    Michael Schrader

  • Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, USA

    Lloyd Fricker

Bibliographic Information

  • Book Title: Peptidomics

  • Book Subtitle: Methods and Strategies

  • Editors: Michael Schrader, Lloyd Fricker

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-7537-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC 2018

  • Hardcover ISBN: 978-1-4939-7536-5Published: 24 February 2018

  • Softcover ISBN: 978-1-4939-8514-2Published: 04 June 2019

  • eBook ISBN: 978-1-4939-7537-2Published: 23 February 2018

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVII, 423

  • Number of Illustrations: 62 b/w illustrations, 17 illustrations in colour

  • Topics: Protein Science

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